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- W2017854183 abstract "We have studied cyclopiazonic acid (CPA)-sensitive store-operated Ca2+ entry (SOCE) in cultured neurons and astrocytes and examined the effect of 2-[2-[4-(4-nitrobenzyloxy)phenyl]]isothiourea (KB-R7943), which is often used as a selective inhibitor of the Na+–Ca2+ exchanger (NCX), on the SOCE. CPA increased transiently intracellular Ca2+ concentration ([Ca2+]i) followed by a sustained increase in [Ca2+]i in neurons and astrocytes. The sustained increase in [Ca2+]i depended on the presence of extracellular Ca2+ and inhibited by SOCE inhibitors, but not by a Ca2+ channel inhibitor. CPA also caused quenching of fura-2 fluorescence when the cells were incubated in Mn2+-containing medium. KB-R7943 at 10 μM inhibited significantly CPA-induced sustained increase in [Ca2+]i in neurons and astrocytes. KB-R7943 also inhibited CPA-induced quenching of fura-2 fluorescence in the presence of extracellular Mn2+. These results indicate that cultured neurons and astrocytes possess SOCE and that KB-R7943 inhibits not only NCX but also SOCE." @default.
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- W2017854183 date "1995-01-01" @default.
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- W2017854183 title "Cyclic nucleotides in the modulation of atrial natriuretic peptide secretion" @default.
- W2017854183 doi "https://doi.org/10.1016/1043-6618(95)87089-x" @default.
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