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- W2017856103 abstract "Isocapnic O2 equilibrium curves (O2EC) were generated for winter hibernating and summer active squirrels (Spermophilus lateralis) at 7 ° and 37 ° C using thin blood film techniques. Half-saturation PO2 at 7 ° C and pHa 7.46 were 5.8±0.1 and 6.9±0.2 Torr for hibernating and summer squirrels, respectively; P50 values at 37 ° C and pHa 7.49 were 15.3|±0.1 and 18.1±0.5 Torr, respectively. This increased blood O2 affinity in the winter animal results, in part, from reductions of RBC organic phosphates. The molar ratio ([ATP] + [DPG])/[Hb4] decreased from 1.55 in summer squirrels to 0.91 in winter hibernators. O2EC shape and CO2 Bohr effect were similar for the two animal groups, but varied with blood temperature. At 7 ° c, Hill plots were nonlinear; Hill's increased from values of 2.2−2.4 below 40% S to 2.7−2.9 above 60% S. At 37 ° C, Hill plots were reasonably linear (n = 25). CO2 Bohr slopes (δlogP50/δpH) for hibernating and euthermic squirrels were −0.37 ± 0.02 and −0.40 ± 0.03 at 7 ° C, respectively, and −0.62 ± 0.04 and −0.60 ± 0.02 at 37 ° C, respectively. Blood O2 capacity was significantly greater (P<0.001) in the hibernator; hematocrit (55%) and [Hb] (19.1 g/dl) exceeded the summer squirrel values by 20% and 25%, respectively. Estimated PVO2 values for summer and winter animals at 7 ° C and pH 7.46 were 7.25 and 6.94 Torr, respectively. This suggests that the effect of increased Hb-O2 affinity on PVO2 is offset by increased circulating [Hb]. We conclude that seasonal changes in the O2 transport properties of squirrel blood do not contribute to the depression of aerobic metabolism during winter hibernation." @default.
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- W2017856103 title "Effects of hibernation on blood oxygen transport in the golden-mantled ground squirrel" @default.
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- W2017856103 doi "https://doi.org/10.1016/0034-5687(94)90116-3" @default.
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