FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017887003> ?p ?o ?g. }

• W2017887003 endingPage "8804" @default.
• W2017887003 startingPage "8801" @default.
• W2017887003 abstract "In previous work, a xeroderma pigmentosum cell line belonging to complementation group C was established by transformation with origin-defective simian virus 40. We now report the complementation of the UV sensitivity of this cell line by gene transfer. A human cDNA clone library constructed in a mammalian expression vector, and itself incorporated in a lambda phage vector, was introduced into the cells as a calcium phosphate precipitate. Following selection to G418 resistance, provided by the neo gene of the vector, transformants were selected for UV resistance. Twenty-one cell clones were obtained with UV-resistance levels typical of normal human fibroblasts. All transformants contained vector DNA sequences in their nuclei. Upon further propagation in the absence of selection for G418 resistance, about half of the primary transformants remained UV-resistant. Secondary transformants were generated by transfection with a partial digest of total chromosomal DNA from one of these stable transformants. This resulted in 15 G418-resistant clones, 2 of which exhibited a UV-resistant phenotype. The other primary clones lost UV resistance rapidly when subcultured in the absence of G418. Importantly, several retained UV resistance under G418 selection pressure. The acquisition of UV resistance by secondary transformants derived by transfection of DNA from a stable primary transformant, and the linkage between G418 and UV resistances in the unstable primary transformants, strongly suggests that the transformants acquired UV resistance through DNA-mediated gene transfer and not by reversion." @default.
• W2017887003 created "2016-06-24" @default.
• W2017887003 creator A5008951145 @default.
• W2017887003 creator A5041342670 @default.
• W2017887003 creator A5047233263 @default.
• W2017887003 creator A5065320270 @default.
• W2017887003 creator A5079266800 @default.
• W2017887003 creator A5091688309 @default.
• W2017887003 date "1987-12-01" @default.
• W2017887003 modified "2023-09-27" @default.
• W2017887003 title "Complementation of the UV-sensitive phenotype of a xeroderma pigmentosum human cell line by transfection with a cDNA clone library." @default.
• W2017887003 cites W1494171325 @default.
• W2017887003 cites W1767349326 @default.
• W2017887003 cites W1918119607 @default.
• W2017887003 cites W1965372133 @default.
• W2017887003 cites W1967446284 @default.
• W2017887003 cites W1969394948 @default.
• W2017887003 cites W1986911839 @default.
• W2017887003 cites W1993813499 @default.
• W2017887003 cites W2000163002 @default.
• W2017887003 cites W2014669590 @default.
• W2017887003 cites W2018299695 @default.
• W2017887003 cites W2027411626 @default.
• W2017887003 cites W2028668677 @default.
• W2017887003 cites W2033819427 @default.
• W2017887003 cites W2057641600 @default.
• W2017887003 cites W2063450941 @default.
• W2017887003 cites W2066352920 @default.
• W2017887003 cites W2086955288 @default.
• W2017887003 cites W2087572810 @default.
• W2017887003 cites W2088072564 @default.
• W2017887003 cites W2113208052 @default.
• W2017887003 cites W2118929543 @default.
• W2017887003 cites W2119658359 @default.
• W2017887003 cites W2152805476 @default.
• W2017887003 cites W2166182249 @default.
• W2017887003 doi "https://doi.org/10.1073/pnas.84.24.8801" @default.
• W2017887003 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/299638" @default.
• W2017887003 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3480511" @default.
• W2017887003 hasPublicationYear "1987" @default.
• W2017887003 type Work @default.
• W2017887003 sameAs 2017887003 @default.
• W2017887003 citedByCount "20" @default.
• W2017887003 countsByYear W20178870032013 @default.
• W2017887003 crossrefType "journal-article" @default.
• W2017887003 hasAuthorship W2017887003A5008951145 @default.
• W2017887003 hasAuthorship W2017887003A5041342670 @default.
• W2017887003 hasAuthorship W2017887003A5047233263 @default.
• W2017887003 hasAuthorship W2017887003A5065320270 @default.
• W2017887003 hasAuthorship W2017887003A5079266800 @default.
• W2017887003 hasAuthorship W2017887003A5091688309 @default.
• W2017887003 hasBestOaLocation W20178870031 @default.
• W2017887003 hasConcept C104317684 @default.
• W2017887003 hasConcept C127716648 @default.
• W2017887003 hasConcept C134935766 @default.
• W2017887003 hasConcept C153911025 @default.
• W2017887003 hasConcept C187882448 @default.
• W2017887003 hasConcept C188082640 @default.
• W2017887003 hasConcept C189482833 @default.
• W2017887003 hasConcept C204241405 @default.
• W2017887003 hasConcept C2778378917 @default.
• W2017887003 hasConcept C54009773 @default.
• W2017887003 hasConcept C54355233 @default.
• W2017887003 hasConcept C552990157 @default.
• W2017887003 hasConcept C81089528 @default.
• W2017887003 hasConcept C81885089 @default.
• W2017887003 hasConcept C86803240 @default.
• W2017887003 hasConceptScore W2017887003C104317684 @default.
• W2017887003 hasConceptScore W2017887003C127716648 @default.
• W2017887003 hasConceptScore W2017887003C134935766 @default.
• W2017887003 hasConceptScore W2017887003C153911025 @default.
• W2017887003 hasConceptScore W2017887003C187882448 @default.
• W2017887003 hasConceptScore W2017887003C188082640 @default.
• W2017887003 hasConceptScore W2017887003C189482833 @default.
• W2017887003 hasConceptScore W2017887003C204241405 @default.
• W2017887003 hasConceptScore W2017887003C2778378917 @default.
• W2017887003 hasConceptScore W2017887003C54009773 @default.
• W2017887003 hasConceptScore W2017887003C54355233 @default.
• W2017887003 hasConceptScore W2017887003C552990157 @default.
• W2017887003 hasConceptScore W2017887003C81089528 @default.
• W2017887003 hasConceptScore W2017887003C81885089 @default.
• W2017887003 hasConceptScore W2017887003C86803240 @default.
• W2017887003 hasIssue "24" @default.
• W2017887003 hasLocation W20178870031 @default.
• W2017887003 hasLocation W20178870032 @default.
• W2017887003 hasLocation W20178870033 @default.
• W2017887003 hasLocation W20178870034 @default.
• W2017887003 hasOpenAccess W2017887003 @default.
• W2017887003 hasPrimaryLocation W20178870031 @default.
• W2017887003 hasRelatedWork W2025166772 @default.
• W2017887003 hasRelatedWork W2074171439 @default.
• W2017887003 hasRelatedWork W2123186148 @default.
• W2017887003 hasRelatedWork W2132936862 @default.
• W2017887003 hasRelatedWork W2290989990 @default.
• W2017887003 hasRelatedWork W2328623076 @default.
• W2017887003 hasRelatedWork W2365832371 @default.
• W2017887003 hasRelatedWork W2379152510 @default.
• W2017887003 hasRelatedWork W2383624830 @default.

• next