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- W2017912912 abstract "Analysis of the ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activase gene and gene products from Arabidopsis thaliana wild-type plants and the Rubisco activase-deficient mutant strain showed that the rca mutation caused GT to be changed to AT at the 5'-splice junction of intron 3 in the six-intron pre-mRNA. Northern blot analysis, genomic and cDNA sequencing, and primer extension analysis indicated that the mutation causes inefficient and incomplete splicing of the pre-mRNA, resulting in the accumulation of three aberrant mRNAs. One mutant mRNA was identical with wild-type mRNA except that it included intron 3, a second mRNA comprised intron 3 and exons 4 through 7, and the third mRNA contained exons 1 through 3. The G-to-A transition is consistent with the known mechanism of mutagenesis by ethyl methanesulfonate, the mutagen used to create the Rubisco activase-deficient strain." @default.
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- W2017912912 title "Molecular Basis of the Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Activase Mutation in Arabidopsis thaliana Is a Guanine-to-Adenine Transition at the 5[prime]-Splice Junction of Intron 3" @default.
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- W2017912912 doi "https://doi.org/10.1104/pp.102.1.227" @default.
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