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- W2017965548 abstract "Human induced pluripotent stem cells (hiPSs) hold great promises in the field of cardiac regeneration, as it possesses the unquestioned potential to differentiate into genuine cardiomyocytes. Though it was proved that hiPSs are able to be differentiated into cardiomycytes, their safety issue for regenerative purposes remain to be unclear. Human embryonic stem cell-derived cardiomyocytes (hESC-CMs), like fetal cardiomyocytes, are electrophysiologically immature and exhibit automaticity. Since proper Ca2+ handling properties of the ES-derived CMs are required for their functional integration with the host tissue, therefore in present study, we used H7 hESC-CMs and IMR90-iPS-CMs to investigate and compare electrophysiological maturation driven by an intrinsic developmental clock/memory with respect to calcium handling properties. Beating embryoid bodies (EB) were first appeared on day 15. As revealed by quantitative RT-PCR, the differentiated iPSCs showed down regulation of cardiac gene, Nkx2.5, α-MHC and β-MHC. Moreover, Ca2+ induced Ca2+ release (CICR) from the sarcoplasmic recticulum (SR) via ryanodine recptor (RyR) is essential in excitation-contraction (EC) coupling as a functional hallmark of adult cardiomyocytes. In junctional SR membrane of cardiomycytes, ryanodine recptor 2 (RyR2) forms inhibitory macrocomplex with junction (Jn), triadin (Trd) and calsequestrin 2 (CASQ2). In hiPS, quantitative RT-PCR revealed significantly down regulation sodium-calcium exchanger (NCX-1) and tridin (Trd), which probably reduces activity of RyR2 activity in hiPSCs, but not affected at transcriptional level. This might account for the decline in calcium release from SR due to reduced activity of spontaneous and caffeine-induced calcium transient as measured by fluorescence confocal imaging carried out in the 2-week-old EBs. In conclusion, hiPSCs showed immature phenotype of calcium handling which implied safety caution had to be taken for the usage of hiPSCs in cardiac regeneration." @default.
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- W2017965548 date "2010-11-01" @default.
- W2017965548 modified "2023-10-04" @default.
- W2017965548 title "P79. Calcium handling in human induced pluripotent stem cells" @default.
- W2017965548 doi "https://doi.org/10.1016/j.diff.2010.09.085" @default.
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