FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2017990787> ?p ?o ?g. }

• W2017990787 endingPage "1158" @default.
• W2017990787 startingPage "1151" @default.
• W2017990787 abstract "Biotransformation of colchicine into regiospecific 3-demethylated colchicine (3-DMC) which is pharmacologically active and a potent anti-cancer drug, mediated by immobilization of recombinant microbial monooxygenases is a novel and promising strategy for its production. In the present study, recombinant Escherichia coli expressing P450 BM-3 was immobilized in calcium-alginate beads and its ability to catalyze colchicine into 3-DMC was investigated. Characteristics of immobilized system showed that optimum conditions for activity of microbial cells were not affected due to immobilization. The optimum pH and temperature for both free and immobilized cells were found to be 7.5 and 37.5 °C, respectively. Experimental variables under consideration such as Ca2+ concentration, alginate concentration, P450 BM-3 enzyme activity and colchicine concentration were optimized using response surface methodology. The immobilized cells exhibited a markedly improved thermal stability as compared to free cells. The yield of 3-DMC with immobilized microbial cells was found to be an average of 69%, with 82, 73 and 52% across three independent batches in succession as against bioconversion by free cells, which indicated improved operational stability and reusability of immobilized cells in batch processes. Additionally, a packed bed reactor has been proposed for the immobilized biocatalytic system for bioconversion of colchicine and other biochemicals." @default.
• W2017990787 created "2016-06-24" @default.
• W2017990787 creator A5016183893 @default.
• W2017990787 creator A5037735602 @default.
• W2017990787 creator A5053008837 @default.
• W2017990787 date "2013-08-01" @default.
• W2017990787 modified "2023-09-23" @default.
• W2017990787 title "Enhanced bioconversion of colchicine to regiospecific 3-demethylated colchicine (3-DMC) by whole cell immobilization of recombinant E. coli harboring P450 BM-3 gene" @default.
• W2017990787 cites W1509833878 @default.
• W2017990787 cites W1535034058 @default.
• W2017990787 cites W1618585289 @default.
• W2017990787 cites W1971710913 @default.
• W2017990787 cites W1980170591 @default.
• W2017990787 cites W1981189407 @default.
• W2017990787 cites W1984007434 @default.
• W2017990787 cites W2001569744 @default.
• W2017990787 cites W2001706065 @default.
• W2017990787 cites W2009564870 @default.
• W2017990787 cites W2011097327 @default.
• W2017990787 cites W2018601250 @default.
• W2017990787 cites W2028576266 @default.
• W2017990787 cites W2046435325 @default.
• W2017990787 cites W2057600655 @default.
• W2017990787 cites W2058894959 @default.
• W2017990787 cites W2064269158 @default.
• W2017990787 cites W2066556063 @default.
• W2017990787 cites W2088061719 @default.
• W2017990787 cites W2088131852 @default.
• W2017990787 cites W2090535274 @default.
• W2017990787 cites W2094969706 @default.
• W2017990787 cites W2095437447 @default.
• W2017990787 cites W2138057980 @default.
• W2017990787 cites W2165811356 @default.
• W2017990787 cites W2409304886 @default.
• W2017990787 cites W2289940340 @default.
• W2017990787 cites W30820233 @default.
• W2017990787 doi "https://doi.org/10.1016/j.procbio.2013.06.001" @default.
• W2017990787 hasPublicationYear "2013" @default.
• W2017990787 type Work @default.
• W2017990787 sameAs 2017990787 @default.
• W2017990787 citedByCount "11" @default.
• W2017990787 countsByYear W20179907872013 @default.
• W2017990787 countsByYear W20179907872015 @default.
• W2017990787 countsByYear W20179907872016 @default.
• W2017990787 countsByYear W20179907872017 @default.
• W2017990787 countsByYear W20179907872018 @default.
• W2017990787 countsByYear W20179907872020 @default.
• W2017990787 countsByYear W20179907872021 @default.
• W2017990787 crossrefType "journal-article" @default.
• W2017990787 hasAuthorship W2017990787A5016183893 @default.
• W2017990787 hasAuthorship W2017990787A5037735602 @default.
• W2017990787 hasAuthorship W2017990787A5053008837 @default.
• W2017990787 hasConcept C100544194 @default.
• W2017990787 hasConcept C104317684 @default.
• W2017990787 hasConcept C134121241 @default.
• W2017990787 hasConcept C161790260 @default.
• W2017990787 hasConcept C178790620 @default.
• W2017990787 hasConcept C181199279 @default.
• W2017990787 hasConcept C185592680 @default.
• W2017990787 hasConcept C191503008 @default.
• W2017990787 hasConcept C191897082 @default.
• W2017990787 hasConcept C192562407 @default.
• W2017990787 hasConcept C2776636253 @default.
• W2017990787 hasConcept C2778713300 @default.
• W2017990787 hasConcept C2780074830 @default.
• W2017990787 hasConcept C27881333 @default.
• W2017990787 hasConcept C40767141 @default.
• W2017990787 hasConcept C43617362 @default.
• W2017990787 hasConcept C519063684 @default.
• W2017990787 hasConcept C54355233 @default.
• W2017990787 hasConcept C547475151 @default.
• W2017990787 hasConcept C55493867 @default.
• W2017990787 hasConcept C65024703 @default.
• W2017990787 hasConcept C86803240 @default.
• W2017990787 hasConceptScore W2017990787C100544194 @default.
• W2017990787 hasConceptScore W2017990787C104317684 @default.
• W2017990787 hasConceptScore W2017990787C134121241 @default.
• W2017990787 hasConceptScore W2017990787C161790260 @default.
• W2017990787 hasConceptScore W2017990787C178790620 @default.
• W2017990787 hasConceptScore W2017990787C181199279 @default.
• W2017990787 hasConceptScore W2017990787C185592680 @default.
• W2017990787 hasConceptScore W2017990787C191503008 @default.
• W2017990787 hasConceptScore W2017990787C191897082 @default.
• W2017990787 hasConceptScore W2017990787C192562407 @default.
• W2017990787 hasConceptScore W2017990787C2776636253 @default.
• W2017990787 hasConceptScore W2017990787C2778713300 @default.
• W2017990787 hasConceptScore W2017990787C2780074830 @default.
• W2017990787 hasConceptScore W2017990787C27881333 @default.
• W2017990787 hasConceptScore W2017990787C40767141 @default.
• W2017990787 hasConceptScore W2017990787C43617362 @default.
• W2017990787 hasConceptScore W2017990787C519063684 @default.
• W2017990787 hasConceptScore W2017990787C54355233 @default.
• W2017990787 hasConceptScore W2017990787C547475151 @default.
• W2017990787 hasConceptScore W2017990787C55493867 @default.
• W2017990787 hasConceptScore W2017990787C65024703 @default.
• W2017990787 hasConceptScore W2017990787C86803240 @default.
• W2017990787 hasIssue "8" @default.
• W2017990787 hasLocation W20179907871 @default.

• next