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• W2017995982 abstract "An in situ hybridization protocol for detecting mRNAs of ostreid herpesvirus type 1 (OsHV-1) which infects Pacific oysters, Crassostrea gigas, was developed. Three RNA probes were synthesized by cloning three partial OsHV-1 genes into plasmids using three specific primer pairs, and performing a transcription in the presence of digoxigenin dUTP. The RNA probes were able to detect the virus mRNAs in paraffin sections of experimentally infected oysters 26 h post-injection. The in situ hybridization showed that the OsHV-1 mRNAs were mainly present in connective tissues in gills, mantle, adductor muscle, digestive gland and gonads. DNA detection by in situ hybridization using a DNA probe and viral DNA quantitation by real-time PCR were also performed and results were compared with those obtained using RNA probes." @default.
• W2017995982 created "2016-06-24" @default.
• W2017995982 creator A5027911259 @default.
• W2017995982 creator A5067316534 @default.
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• W2017995982 creator A5086077496 @default.
• W2017995982 date "2015-01-01" @default.
• W2017995982 modified "2023-10-09" @default.
• W2017995982 title "Development of an in situ hybridization assay for the detection of ostreid herpesvirus type 1 mRNAs in the Pacific oyster, Crassostrea gigas" @default.
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• W2017995982 doi "https://doi.org/10.1016/j.jviromet.2014.10.007" @default.
• W2017995982 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25455903" @default.
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