Matches in SemOpenAlex for { <https://semopenalex.org/work/W2018013580> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2018013580 abstract "In order to assess how lever arm length affects the three-dimensional motions of myosin V during processive motility, two constructs were studied using single molecule polarized total internal reflection fluorescence (polTIRF) microscopy. MyoV6IQ and MyoV4IQ contain 6 and 4 calmodulin (CaM) binding IQ motifs, and otherwise consist of the native myosin V excluding the tail domain. Bifunctional rhodamine labeled CaM replaced a native CaM, giving probe angles βP relative to the actin axis and αP, the azimuth around actin. As with other processive myosins, αP and βP exhibited tilting of the probe with each step. With MyoV6IQ, αP often returned to its initial value after two steps, as expected for nearly straight walking. This behavior enabled us to determine the orientation of the lever arm, αL and βL, as well as θL and fL, the probe angles relative to CaM. βL was 100° and 40° in the leading and trailing heads, respectively. In MyoV4IQ, βP was similar to 6IQ, but αP seldom returned to its earlier value after two steps. This indicates considerable net azimuthal rotation, as expected for smaller step sizes. Thus, lever arm length determines the azimuthal angular path, whereas the axial orientation is likely determined by structural constraints in the motor domain. Modified gliding filament assays were performed using polTIRF to detect twirling of actin about its axis during motility. MyoV6IQ twirled almost exclusively left-handed with a pitch of 1.4 μm. MyoV4IQ twirled with both right- and left-handed pitches of 1.0 and 1.2 μm, respectively. Bidirectional twirling of MyoV4IQ contrasts with every isoform of myosin previously tested (II, native V, VI and X) all of which twirled with a single handedness. This work was supported by NIH grant AR05117." @default.
- W2018013580 created "2016-06-24" @default.
- W2018013580 creator A5002499186 @default.
- W2018013580 creator A5013832205 @default.
- W2018013580 creator A5042769152 @default.
- W2018013580 creator A5065112291 @default.
- W2018013580 date "2009-02-01" @default.
- W2018013580 modified "2023-09-27" @default.
- W2018013580 title "Lever Arm Length Determines The Azimuthal But Not The Axial Orientation Of Myosin V During Processive Motility" @default.
- W2018013580 doi "https://doi.org/10.1016/j.bpj.2008.12.3882" @default.
- W2018013580 hasPublicationYear "2009" @default.
- W2018013580 type Work @default.
- W2018013580 sameAs 2018013580 @default.
- W2018013580 citedByCount "0" @default.
- W2018013580 crossrefType "journal-article" @default.
- W2018013580 hasAuthorship W2018013580A5002499186 @default.
- W2018013580 hasAuthorship W2018013580A5013832205 @default.
- W2018013580 hasAuthorship W2018013580A5042769152 @default.
- W2018013580 hasAuthorship W2018013580A5065112291 @default.
- W2018013580 hasBestOaLocation W20180135801 @default.
- W2018013580 hasConcept C105702510 @default.
- W2018013580 hasConcept C107524782 @default.
- W2018013580 hasConcept C120665830 @default.
- W2018013580 hasConcept C121332964 @default.
- W2018013580 hasConcept C12554922 @default.
- W2018013580 hasConcept C125705527 @default.
- W2018013580 hasConcept C14228908 @default.
- W2018013580 hasConcept C159737794 @default.
- W2018013580 hasConcept C16345878 @default.
- W2018013580 hasConcept C185592680 @default.
- W2018013580 hasConcept C2524010 @default.
- W2018013580 hasConcept C33923547 @default.
- W2018013580 hasConcept C55493867 @default.
- W2018013580 hasConcept C58207958 @default.
- W2018013580 hasConcept C62520636 @default.
- W2018013580 hasConcept C6997183 @default.
- W2018013580 hasConcept C74050887 @default.
- W2018013580 hasConcept C86803240 @default.
- W2018013580 hasConcept C95444343 @default.
- W2018013580 hasConceptScore W2018013580C105702510 @default.
- W2018013580 hasConceptScore W2018013580C107524782 @default.
- W2018013580 hasConceptScore W2018013580C120665830 @default.
- W2018013580 hasConceptScore W2018013580C121332964 @default.
- W2018013580 hasConceptScore W2018013580C12554922 @default.
- W2018013580 hasConceptScore W2018013580C125705527 @default.
- W2018013580 hasConceptScore W2018013580C14228908 @default.
- W2018013580 hasConceptScore W2018013580C159737794 @default.
- W2018013580 hasConceptScore W2018013580C16345878 @default.
- W2018013580 hasConceptScore W2018013580C185592680 @default.
- W2018013580 hasConceptScore W2018013580C2524010 @default.
- W2018013580 hasConceptScore W2018013580C33923547 @default.
- W2018013580 hasConceptScore W2018013580C55493867 @default.
- W2018013580 hasConceptScore W2018013580C58207958 @default.
- W2018013580 hasConceptScore W2018013580C62520636 @default.
- W2018013580 hasConceptScore W2018013580C6997183 @default.
- W2018013580 hasConceptScore W2018013580C74050887 @default.
- W2018013580 hasConceptScore W2018013580C86803240 @default.
- W2018013580 hasConceptScore W2018013580C95444343 @default.
- W2018013580 hasLocation W20180135801 @default.
- W2018013580 hasOpenAccess W2018013580 @default.
- W2018013580 hasPrimaryLocation W20180135801 @default.
- W2018013580 hasRelatedWork W1964533978 @default.
- W2018013580 hasRelatedWork W1967237859 @default.
- W2018013580 hasRelatedWork W1976138338 @default.
- W2018013580 hasRelatedWork W1985871124 @default.
- W2018013580 hasRelatedWork W1995171216 @default.
- W2018013580 hasRelatedWork W2011750347 @default.
- W2018013580 hasRelatedWork W2013361751 @default.
- W2018013580 hasRelatedWork W2015404233 @default.
- W2018013580 hasRelatedWork W2017085405 @default.
- W2018013580 hasRelatedWork W2024130725 @default.
- W2018013580 hasRelatedWork W2030769440 @default.
- W2018013580 hasRelatedWork W2073784091 @default.
- W2018013580 hasRelatedWork W2074655461 @default.
- W2018013580 hasRelatedWork W2078241323 @default.
- W2018013580 hasRelatedWork W2082482266 @default.
- W2018013580 hasRelatedWork W2086748294 @default.
- W2018013580 hasRelatedWork W2097766254 @default.
- W2018013580 hasRelatedWork W2128994323 @default.
- W2018013580 hasRelatedWork W2138198227 @default.
- W2018013580 hasRelatedWork W2139995627 @default.
- W2018013580 isParatext "false" @default.
- W2018013580 isRetracted "false" @default.
- W2018013580 magId "2018013580" @default.
- W2018013580 workType "article" @default.