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- W2018071451 abstract "Real-time PCR assays were developed to quantitate Mycobacterium avium subsp. paratuberculosis (MAP) in bovine monocyte-derived macrophages. We measured the absolute number of both host cells and bacteria in in vitro challenge assays. Results obtained from real-time quantitative PCR (qPCR) DNA copy counts were compared to visual quantitation of fluorescent-stained MAP in macrophages. Conclusions from our original visual analysis were supported by the second (qPCR) methodology; however, the qPCR assay proved to be more consistent between samples and was easier to perform. There was a strain-to-strain difference in growth curves between fluorescent quantitation (FQ) and qPCR that we believe to be a consequence of bacterial growth characteristics in FQ. In summary, real-time PCR assays provided a more accurate and precise method for evaluating intracellular growth dynamics when comparing strains of MAP." @default.
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- W2018071451 date "2011-01-01" @default.
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- W2018071451 title "Quantification of Mycobacterium avium subsp. paratuberculosis (MAP) survival in monocyte-derived macrophages" @default.
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- W2018071451 doi "https://doi.org/10.1016/j.vetimm.2010.08.003" @default.
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