FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2018077753> ?p ?o ?g. }

• W2018077753 endingPage "490" @default.
• W2018077753 startingPage "480" @default.
• W2018077753 abstract "The present study describes an embryonic-fetal liver culture system which allows morphogenetic interactions consistent with the development of the hepatocellular function.Intact livers from 8-12-week embryos were soaked in an extracellular matrix at 4 degrees C and gently dissociated without any enzymatic treatment. The resulting spherical hepatic units were cultured in a chemically defined serum-free medium and seeded into an extracellular matrix layer. Adherent three-dimensional tissue specimens were examined at various times by light and electron microscopy to evaluate the maintenance of hepatocyte morphology.The liver cells were viable for over 4 months; erythropoietic burst colonies were detected for longer than 6 weeks. Parallel detection of bile salt production in the medium by high performance liquid chromatography proved liver tissue functionality. Bile salt composition revealed predominance of taurine-conjugates rather than glycine. Maximum bile salt concentration (approximately 3 months) coincided with structural and ultrastructural observations indicating a marked decline in hematopoiesis, well-defined biliary canaliculi and formation of an organ-like structure.This three-dimensional culture system recapitulates fetal liver development with: (i) initial proliferation of both fetal erythropoietic and hepatic cells and (ii) subsequent shut-off of erythropoiesis and a shift to a more advanced stage of hepatocyte function, such as bile salt secretion." @default.
• W2018077753 created "2016-06-24" @default.
• W2018077753 creator A5031243945 @default.
• W2018077753 creator A5032499206 @default.
• W2018077753 creator A5042442375 @default.
• W2018077753 creator A5044241488 @default.
• W2018077753 creator A5046002870 @default.
• W2018077753 creator A5057239346 @default.
• W2018077753 creator A5059963876 @default.
• W2018077753 creator A5065595032 @default.
• W2018077753 date "1998-03-01" @default.
• W2018077753 modified "2023-09-23" @default.
• W2018077753 title "Long-term cultures of human fetal liver cells: a three-dimensional experimental model for monitoring liver tissue development" @default.
• W2018077753 cites W1495986809 @default.
• W2018077753 cites W1532411911 @default.
• W2018077753 cites W1549334240 @default.
• W2018077753 cites W1619527311 @default.
• W2018077753 cites W1868395294 @default.
• W2018077753 cites W1963935096 @default.
• W2018077753 cites W1968053028 @default.
• W2018077753 cites W1974096743 @default.
• W2018077753 cites W1975522922 @default.
• W2018077753 cites W1976952335 @default.
• W2018077753 cites W1977665282 @default.
• W2018077753 cites W1983524666 @default.
• W2018077753 cites W1988300335 @default.
• W2018077753 cites W1995619290 @default.
• W2018077753 cites W2002783109 @default.
• W2018077753 cites W2005209749 @default.
• W2018077753 cites W2005638713 @default.
• W2018077753 cites W2007496421 @default.
• W2018077753 cites W2008434851 @default.
• W2018077753 cites W2019477145 @default.
• W2018077753 cites W2022217882 @default.
• W2018077753 cites W2022527563 @default.
• W2018077753 cites W2025089934 @default.
• W2018077753 cites W2031896298 @default.
• W2018077753 cites W2033581462 @default.
• W2018077753 cites W2035849514 @default.
• W2018077753 cites W2041773938 @default.
• W2018077753 cites W2054044046 @default.
• W2018077753 cites W2058153935 @default.
• W2018077753 cites W2062602912 @default.
• W2018077753 cites W2075119774 @default.
• W2018077753 cites W2088468259 @default.
• W2018077753 cites W2095620987 @default.
• W2018077753 cites W2100543326 @default.
• W2018077753 cites W2103385168 @default.
• W2018077753 cites W2108102256 @default.
• W2018077753 cites W2129849944 @default.
• W2018077753 cites W2141979812 @default.
• W2018077753 doi "https://doi.org/10.1016/s0168-8278(98)80323-1" @default.
• W2018077753 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9551687" @default.
• W2018077753 hasPublicationYear "1998" @default.
• W2018077753 type Work @default.
• W2018077753 sameAs 2018077753 @default.
• W2018077753 citedByCount "4" @default.
• W2018077753 countsByYear W20180777532014 @default.
• W2018077753 crossrefType "journal-article" @default.
• W2018077753 hasAuthorship W2018077753A5031243945 @default.
• W2018077753 hasAuthorship W2018077753A5032499206 @default.
• W2018077753 hasAuthorship W2018077753A5042442375 @default.
• W2018077753 hasAuthorship W2018077753A5044241488 @default.
• W2018077753 hasAuthorship W2018077753A5046002870 @default.
• W2018077753 hasAuthorship W2018077753A5057239346 @default.
• W2018077753 hasAuthorship W2018077753A5059963876 @default.
• W2018077753 hasAuthorship W2018077753A5065595032 @default.
• W2018077753 hasConcept C105702510 @default.
• W2018077753 hasConcept C126322002 @default.
• W2018077753 hasConcept C134018914 @default.
• W2018077753 hasConcept C142724271 @default.
• W2018077753 hasConcept C16685009 @default.
• W2018077753 hasConcept C172680121 @default.
• W2018077753 hasConcept C176891718 @default.
• W2018077753 hasConcept C185592680 @default.
• W2018077753 hasConcept C189165786 @default.
• W2018077753 hasConcept C202751555 @default.
• W2018077753 hasConcept C203014093 @default.
• W2018077753 hasConcept C2776200302 @default.
• W2018077753 hasConcept C2776417487 @default.
• W2018077753 hasConcept C2778248108 @default.
• W2018077753 hasConcept C2779234561 @default.
• W2018077753 hasConcept C2779703530 @default.
• W2018077753 hasConcept C54355233 @default.
• W2018077753 hasConcept C55493867 @default.
• W2018077753 hasConcept C71924100 @default.
• W2018077753 hasConcept C86803240 @default.
• W2018077753 hasConcept C94033540 @default.
• W2018077753 hasConcept C95444343 @default.
• W2018077753 hasConceptScore W2018077753C105702510 @default.
• W2018077753 hasConceptScore W2018077753C126322002 @default.
• W2018077753 hasConceptScore W2018077753C134018914 @default.
• W2018077753 hasConceptScore W2018077753C142724271 @default.
• W2018077753 hasConceptScore W2018077753C16685009 @default.
• W2018077753 hasConceptScore W2018077753C172680121 @default.
• W2018077753 hasConceptScore W2018077753C176891718 @default.
• W2018077753 hasConceptScore W2018077753C185592680 @default.
• W2018077753 hasConceptScore W2018077753C189165786 @default.

• next