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- W201810880 abstract "The present report describes an atypical Brucella ovis strain (Bo10) isolated from the epididymis and testis of aninfected ram. Macroscopic and microscopic lesions characteristic for the infection, including positive Brucella immunostaining,were observed within lesions in the genital organs. Compared to other isolates, strain Bo10 required an additional day (atotal of 96 hr) of incubation to form visible colonies, showed a distinct carbon source utilization profile, agglutinated onlyweakly with rough (R) serum, but showed a high capacity for autoagglutination. Isolate Bo10 failed to produce the 1,071-bpfragment in the outer membrane protein (omp) 31 gene–based part of the “Bruce-ladder” multiplex polymerase chain reactionsystem but did produce a 1,915-bp amplicon, thus presenting a profile similar to Brucella abortus. Sequence analysis of the1,915-bp fragment revealed an 842-bp long insertion sequence (IS)711 transposon element inserted into the promoter regionof the omp31 gene, immediately upstream from the ribosome binding site (-10 box/Pribnow box). Sodium dodecyl sulfate–polyacrylamide gel electrophoresis of a whole-cell lysate showed the absence in Bo10 of the approximately 31-kDa proteinfragment associated with omp31. The results demonstrate a natural inactivation of omp31 and, consequently, the absence ofthe Omp31 protein in this B. ovis isolate. The novel location of IS711 within the genome of a naturally occurring B. ovis strainsupports the hypothesis that IS711 could be an active transposon in this Brucella species." @default.
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- W201810880 date "2013-01-01" @default.
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- W201810880 title "Natural IS711 insertion causing omp31gene inactivation in Brucella ovis" @default.
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