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- W2018127725 abstract "Exchange and recombination of chromosomal markers is an intrinsic genetic property of the thermoacidophilic archaeon Sulfolobus acidocaldarius that has not been thoroughly characterized. To clarify the mechanism and experimental usefulness of this process, the frequency of S. acidocaldarius prototrophs produced from mixtures of two pyrimidine auxotrophs under a variety of conditions was determined. The apparent efficiency of genetic exchange was essentially independent of the density of cells deposited on the surface of solid media. Furthermore, recombinant formation could initiate in liquid suspensions, as indicated by high recombinant frequencies resulting from mixtures plated at low cell densities, and the formation of recombinants at equal or higher frequencies in liquid suspensions that were never plated. Apparent initiation of genetic exchange in liquid at 22 °C was not prevented by DNase, prior digestion of parental cells with protease from Streptomyces griseus , or any other non-lethal chemical agent tested. The results support prior indications that chromosomal marker exchange in S. acidocaldarius proceeds via conjugation, and further indicate that this conjugation can initiate quickly in dilute liquid suspension. The mating system of S. acidocaldarius thus appears physiologically distinct from that of Haloferax volcanii but perhaps similar to conjugational transfer of Sulfolobus plasmid pNOB8. The frequency of recombinants formed in these assays (10 -4 -10 -5 per c.f.u.) greatly exceeds the number of spontaneous forward mutational events per generation for biosynthetic genes in S. acidocaldarius. This suggests that chromosomal exchange has the potential to influence the genetic dynamics of natural Sulfolobus populations." @default.
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- W2018127725 title "Chromosomal marker exchange in the thermophilic archaeon Sulfolobus acidocaldarius: physiological and cellular aspects" @default.
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- W2018127725 doi "https://doi.org/10.1099/00221287-144-6-1649" @default.
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