FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2018177015> ?p ?o ?g. }

• W2018177015 endingPage "2135" @default.
• W2018177015 startingPage "2125" @default.
• W2018177015 abstract "Abstract Quantitative proteomics has been widely used to elucidate many cellular processes. In particular, stable isotope labeling by amino acids in cell culture (SILAC) has been instrumental in improving the quality of data generated from quantitative high-throughput proteomic studies. SILAC uses the cell’s natural metabolic pathways to label proteins with isotopically heavy amino acids. Incorporation of these heavy amino acids effectively labels a cell’s proteome, allowing the comparison of cell cultures treated under different conditions. SILAC has been successfully applied to a variety of model organisms including yeast, fruit flies, plants, and mice to look for kinase substrates as well as protein–protein interactions. In budding yeast, several kinases are known to play critical roles in different aspects of meiosis. Therefore, the use of SILAC to identify potential kinase substrates would be helpful in the understanding the specific mechanisms by which these kinases act. Previously, it has not been possible to use SILAC to quantitatively study the phosphoproteome of meiotic Saccharomyces cerevisiae cells, because yeast cells sporulate inefficiently after pregrowth in standard synthetic medium. In this study we report the development of a synthetic, SILAC-compatible, pre-sporulation medium (RPS) that allows for efficient sporulation of S. cerevisiae SK1 diploids. Pre-growth in RPS supplemented with heavy amino acids efficiently labels the proteome, after which cells proceed relatively synchronously through meiosis, producing highly viable spores. As proof of principle, SILAC experiments were able to identify known targets of the meiosis-specific kinase Mek1." @default.
• W2018177015 created "2016-06-24" @default.
• W2018177015 creator A5031897845 @default.
• W2018177015 creator A5045966023 @default.
• W2018177015 creator A5049065304 @default.
• W2018177015 creator A5057471078 @default.
• W2018177015 creator A5074405835 @default.
• W2018177015 date "2014-11-01" @default.
• W2018177015 modified "2023-10-12" @default.
• W2018177015 title "A Method for Sporulating Budding Yeast Cells That Allows for Unbiased Identification of Kinase Substrates Using Stable Isotope Labeling by Amino Acids in Cell Culture" @default.
• W2018177015 cites W1943612516 @default.
• W2018177015 cites W1966072209 @default.
• W2018177015 cites W1968096672 @default.
• W2018177015 cites W1969367585 @default.
• W2018177015 cites W1970404134 @default.
• W2018177015 cites W1971201699 @default.
• W2018177015 cites W1971801697 @default.
• W2018177015 cites W1973507920 @default.
• W2018177015 cites W1997859335 @default.
• W2018177015 cites W2004704197 @default.
• W2018177015 cites W2006331214 @default.
• W2018177015 cites W2010442490 @default.
• W2018177015 cites W2022753514 @default.
• W2018177015 cites W2029856558 @default.
• W2018177015 cites W2036820511 @default.
• W2018177015 cites W2039781354 @default.
• W2018177015 cites W2041802268 @default.
• W2018177015 cites W2047106949 @default.
• W2018177015 cites W2047261627 @default.
• W2018177015 cites W2055193421 @default.
• W2018177015 cites W2056750443 @default.
• W2018177015 cites W2064363195 @default.
• W2018177015 cites W2065271821 @default.
• W2018177015 cites W2070650108 @default.
• W2018177015 cites W2074350748 @default.
• W2018177015 cites W2077705447 @default.
• W2018177015 cites W2078743927 @default.
• W2018177015 cites W2085289028 @default.
• W2018177015 cites W2095953854 @default.
• W2018177015 cites W2096057003 @default.
• W2018177015 cites W2100298442 @default.
• W2018177015 cites W2101380023 @default.
• W2018177015 cites W2111059688 @default.
• W2018177015 cites W2111954050 @default.
• W2018177015 cites W2114171007 @default.
• W2018177015 cites W2118390137 @default.
• W2018177015 cites W2123929534 @default.
• W2018177015 cites W2130760462 @default.
• W2018177015 cites W2133277995 @default.
• W2018177015 cites W2136536970 @default.
• W2018177015 cites W2140946052 @default.
• W2018177015 cites W2144190798 @default.
• W2018177015 cites W2147013899 @default.
• W2018177015 cites W2153820061 @default.
• W2018177015 cites W2166957980 @default.
• W2018177015 cites W2223503042 @default.
• W2018177015 doi "https://doi.org/10.1534/g3.114.013888" @default.
• W2018177015 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4232538" @default.
• W2018177015 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25168012" @default.
• W2018177015 hasPublicationYear "2014" @default.
• W2018177015 type Work @default.
• W2018177015 sameAs 2018177015 @default.
• W2018177015 citedByCount "11" @default.
• W2018177015 countsByYear W20181770152015 @default.
• W2018177015 countsByYear W20181770152016 @default.
• W2018177015 countsByYear W20181770152017 @default.
• W2018177015 countsByYear W20181770152018 @default.
• W2018177015 countsByYear W20181770152021 @default.
• W2018177015 countsByYear W20181770152023 @default.
• W2018177015 crossrefType "journal-article" @default.
• W2018177015 hasAuthorship W2018177015A5031897845 @default.
• W2018177015 hasAuthorship W2018177015A5045966023 @default.
• W2018177015 hasAuthorship W2018177015A5049065304 @default.
• W2018177015 hasAuthorship W2018177015A5057471078 @default.
• W2018177015 hasAuthorship W2018177015A5074405835 @default.
• W2018177015 hasBestOaLocation W20181770151 @default.
• W2018177015 hasConcept C104317684 @default.
• W2018177015 hasConcept C104397665 @default.
• W2018177015 hasConcept C184235292 @default.
• W2018177015 hasConcept C2777576037 @default.
• W2018177015 hasConcept C2779222958 @default.
• W2018177015 hasConcept C34905852 @default.
• W2018177015 hasConcept C46111723 @default.
• W2018177015 hasConcept C515207424 @default.
• W2018177015 hasConcept C55493867 @default.
• W2018177015 hasConcept C6675166 @default.
• W2018177015 hasConcept C70721500 @default.
• W2018177015 hasConcept C80311884 @default.
• W2018177015 hasConcept C86803240 @default.
• W2018177015 hasConcept C87325107 @default.
• W2018177015 hasConcept C95444343 @default.
• W2018177015 hasConcept C97029542 @default.
• W2018177015 hasConceptScore W2018177015C104317684 @default.
• W2018177015 hasConceptScore W2018177015C104397665 @default.
• W2018177015 hasConceptScore W2018177015C184235292 @default.
• W2018177015 hasConceptScore W2018177015C2777576037 @default.
• W2018177015 hasConceptScore W2018177015C2779222958 @default.
• W2018177015 hasConceptScore W2018177015C34905852 @default.

• next