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W2018185922 abstract "For many biotechnological applications the label-free detection of biomolecular interactions is becoming of outstanding importance. In this Article we report the direct electrical detection of small peptides and proteins by their intrinsic charges using a biofunctionalized thin-film resistor. The label-free selective and quantitative detection of small peptides and proteins is achieved using hydrophobized silicon-on-insulator (SOI) substrates functionalized with lipid membranes that incorporate metal-chelating lipids. The response of the nanometer-thin conducting silicon film to electrolyte screening effects is taken into account to determine quantitatively the charges of peptides. It is even possible to detect peptides with a single charge and to distinguish single charge variations of the analytes even in physiological electrolyte solutions. As the device is based on standard semiconductor technologies, parallelization and miniaturization of the SOI-based biosensor is achievable by standard CMOS technologies and thus a promising basis for high-throughput screening or biotechnological applications." @default.
W2018185922 created "2016-06-24" @default.
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W2018185922 date "2006-01-11" @default.
W2018185922 modified "2023-10-13" @default.
W2018185922 title "Field Effect of Screened Charges: Electrical Detection of Peptides and Proteins by a Thin-Film Resistor" @default.
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W2018185922 doi "https://doi.org/10.1002/cphc.200500484" @default.
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