Matches in SemOpenAlex for { <https://semopenalex.org/work/W2018191950> ?p ?o ?g. }
- W2018191950 endingPage "525" @default.
- W2018191950 startingPage "517" @default.
- W2018191950 abstract "Bloom syndrome is a rare genetic disorder characterized by severe growth retardation and cancer predisposition. The disease is caused by a loss of function of the Bloom syndrome protein (BLM), a member of the RecQ family of DNA helicases. Here we report on the first 3D structure of a BLM fragment, a solution structure of the C-terminal helicase-and-ribonuclease D-C-terminal (HRDC) domain from human BLM. The structure reveals unique features of BLM HRDC that are distinct from the HRDC domain of Werner syndrome protein. In particular, BLM HRDC retains many acidic residues exposed to the solvent, which makes the domain surface extensively electronegative. Consistent with this, fluorescence polarization assays showed an inability of isolated BLM HRDC to interact with DNA substrates. Analyses employing ultracentrifugation, gel-filtration, CD spectroscopy and dynamic light scattering showed that the BLM HRDC domain exists as a stable monomer in solution. The results show that BLM HRDC is a compact, robust and acidic motif which may play a distinct role apart from DNA binding." @default.
- W2018191950 created "2016-06-24" @default.
- W2018191950 creator A5004225827 @default.
- W2018191950 creator A5015092891 @default.
- W2018191950 creator A5022207558 @default.
- W2018191950 creator A5033361967 @default.
- W2018191950 creator A5043514474 @default.
- W2018191950 creator A5056977330 @default.
- W2018191950 creator A5068049306 @default.
- W2018191950 creator A5085302536 @default.
- W2018191950 date "2010-08-25" @default.
- W2018191950 modified "2023-09-25" @default.
- W2018191950 title "Solution structure of the HRDC domain of human Bloom syndrome protein BLM" @default.
- W2018191950 cites W1589295940 @default.
- W2018191950 cites W1721339034 @default.
- W2018191950 cites W1744821831 @default.
- W2018191950 cites W1968676497 @default.
- W2018191950 cites W1975459052 @default.
- W2018191950 cites W1976194927 @default.
- W2018191950 cites W1984989145 @default.
- W2018191950 cites W1995017064 @default.
- W2018191950 cites W1996122311 @default.
- W2018191950 cites W2006053315 @default.
- W2018191950 cites W2006322108 @default.
- W2018191950 cites W2038195538 @default.
- W2018191950 cites W2043005203 @default.
- W2018191950 cites W2049357251 @default.
- W2018191950 cites W2050493898 @default.
- W2018191950 cites W2053202752 @default.
- W2018191950 cites W2058486409 @default.
- W2018191950 cites W2069138831 @default.
- W2018191950 cites W2074125914 @default.
- W2018191950 cites W2078383934 @default.
- W2018191950 cites W2082447947 @default.
- W2018191950 cites W2098613471 @default.
- W2018191950 cites W2151037827 @default.
- W2018191950 cites W2160793656 @default.
- W2018191950 cites W2169821755 @default.
- W2018191950 doi "https://doi.org/10.1093/jb/mvq097" @default.
- W2018191950 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20739603" @default.
- W2018191950 hasPublicationYear "2010" @default.
- W2018191950 type Work @default.
- W2018191950 sameAs 2018191950 @default.
- W2018191950 citedByCount "27" @default.
- W2018191950 countsByYear W20181919502012 @default.
- W2018191950 countsByYear W20181919502013 @default.
- W2018191950 countsByYear W20181919502014 @default.
- W2018191950 countsByYear W20181919502015 @default.
- W2018191950 countsByYear W20181919502016 @default.
- W2018191950 countsByYear W20181919502017 @default.
- W2018191950 countsByYear W20181919502019 @default.
- W2018191950 countsByYear W20181919502020 @default.
- W2018191950 countsByYear W20181919502021 @default.
- W2018191950 countsByYear W20181919502022 @default.
- W2018191950 countsByYear W20181919502023 @default.
- W2018191950 crossrefType "journal-article" @default.
- W2018191950 hasAuthorship W2018191950A5004225827 @default.
- W2018191950 hasAuthorship W2018191950A5015092891 @default.
- W2018191950 hasAuthorship W2018191950A5022207558 @default.
- W2018191950 hasAuthorship W2018191950A5033361967 @default.
- W2018191950 hasAuthorship W2018191950A5043514474 @default.
- W2018191950 hasAuthorship W2018191950A5056977330 @default.
- W2018191950 hasAuthorship W2018191950A5068049306 @default.
- W2018191950 hasAuthorship W2018191950A5085302536 @default.
- W2018191950 hasConcept C104317684 @default.
- W2018191950 hasConcept C12554922 @default.
- W2018191950 hasConcept C161223559 @default.
- W2018191950 hasConcept C185592680 @default.
- W2018191950 hasConcept C2779013463 @default.
- W2018191950 hasConcept C55493867 @default.
- W2018191950 hasConcept C67705224 @default.
- W2018191950 hasConcept C8010536 @default.
- W2018191950 hasConcept C86803240 @default.
- W2018191950 hasConceptScore W2018191950C104317684 @default.
- W2018191950 hasConceptScore W2018191950C12554922 @default.
- W2018191950 hasConceptScore W2018191950C161223559 @default.
- W2018191950 hasConceptScore W2018191950C185592680 @default.
- W2018191950 hasConceptScore W2018191950C2779013463 @default.
- W2018191950 hasConceptScore W2018191950C55493867 @default.
- W2018191950 hasConceptScore W2018191950C67705224 @default.
- W2018191950 hasConceptScore W2018191950C8010536 @default.
- W2018191950 hasConceptScore W2018191950C86803240 @default.
- W2018191950 hasIssue "4" @default.
- W2018191950 hasLocation W20181919501 @default.
- W2018191950 hasLocation W20181919502 @default.
- W2018191950 hasOpenAccess W2018191950 @default.
- W2018191950 hasPrimaryLocation W20181919501 @default.
- W2018191950 hasRelatedWork W1984551289 @default.
- W2018191950 hasRelatedWork W2017592458 @default.
- W2018191950 hasRelatedWork W2057859059 @default.
- W2018191950 hasRelatedWork W2105399066 @default.
- W2018191950 hasRelatedWork W2123391682 @default.
- W2018191950 hasRelatedWork W2394127529 @default.
- W2018191950 hasRelatedWork W2767324985 @default.
- W2018191950 hasRelatedWork W4232419554 @default.
- W2018191950 hasRelatedWork W4237282683 @default.
- W2018191950 hasRelatedWork W4281571197 @default.
- W2018191950 hasVolume "148" @default.