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- W2018213035 abstract "In the present study, we describe the cloning and sequence analysis of rat IL-3. Two different mRNA isoforms were isolated after transfection of COS cells with the cytokine genomic sequences. One of the isoforms has been predicted before by Cohen et al. (1986) , and the other one is identical except that it encodes a protein with an insertion of three amino acids at position 56. As names for the two isoforms, we propose IL-3 α for the predicted and IL-3 β for the novel molecule. IL-3 β mRNA was detected as the predominant isoform in rat lymphocytes in vivo. High levels of the cytokine were obtained after infection of human cells (A549) with a recombinant adenovirus harboring rIL-3 β cDNA (IG.Ad.CMV.IL-3 β ). The biological properties of the IL-3 β protein were tested in a FDC-P1 proliferation assay and in a hematopoietic progenitor colony forming assay. To assess in-vivo bioactivity, lysed 293 cells containing IG.Ad.CMV.rIL-3 β virus were injected subcutaneously into F344 rats . Stimulation of hematopoiesis and leucocytosis were observed during the treatment. After subcutaneous injections of the lysed adeno-producer cells in mice, the only effect observed was a cellular infiltration at the site of injection, confirming the poor cross-reactivity between the two species. The biological properties in vitro and in vivo demonstrate that the cDNA sequences of IL-3 β presented here encode active rat IL-3 protein." @default.
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- W2018213035 date "1998-04-01" @default.
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- W2018213035 title "Cloning, biological characterization and high-level expression of rat Interleukin-3 using recombinant adenovirus: description of a new splicing variant" @default.
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- W2018213035 doi "https://doi.org/10.1016/s0378-1119(98)00111-5" @default.
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