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- W2018220370 abstract "A method for the determination of total N-nitroso compounds (NOC) by chemical denitrosation and subsequent chemiluminescence detection of evolved NO is described. Denitrosation was accomplished with CuCl in HCl at 70 °C. The detection limit for N-nitrosoproline (NPRO) was 1 pmol. NO formation from NPRO was linear (R2 = 0.999) from 4 pmol to 2 nmol. Among the possible interfering compounds tested, only S-nitroso compounds contribute any significant interference. This method had several advantages over other similar methods: (1) A commercially available one-piece reaction vessel and a NO analyzer with software were used. (2) NO release occurred rapidly and was easily measured and quantified. (3) Compared to HBr or HI, CuCl was more convenient to work with and safe. (4) CuCl was suitable for samples in aqueous and most organic solvents. The application of this method to food, personal care products, and human body fluids demonstrates its utility. Keywords: Total N-nitroso compounds; chemical denitrosation; chemiluminescence; CuCl; NO; Nitric Oxide Analyzer (NOA 280); Radical Purger" @default.
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- W2018220370 date "2005-05-19" @default.
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- W2018220370 title "Determination of Total <i>N</i>-Nitroso Compounds by Chemical Denitrosation Using CuCl" @default.
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- W2018220370 doi "https://doi.org/10.1021/jf0481709" @default.
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