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- W2018234638 abstract "1. The inhibitory effect of extracts of rat liver on DNA polymerase (deoxyribonucleosidetriphosphate: DNA deoxyribonucleotidyltransferase, EC 2.7.7.7) has been investigated. 2. Fractionation of the crude extracts has revealed that the inhibition is not due to the hydrolysis of the deoxyribonucleoside 5′-triphosphates by phosphatases. 3. The inhibitory factor was purified about 10–15-fold, with a yield of 20%. 4. Its activity was due to the action on the primer DNA of a deoxyribonuclease, and the oligonucleotide products of the reaction inhibited the polymerase. 5. The properties of the deoxyribonuclease differed in some respects from those of splenic deoxyribonuclease II (deoxyribonucleate 3′-nucleotidohydrolase, EC 3.1.4.6) and pancreatic deoxyribonuclease I (deoxyribonucleate oligonucleotidohydrolase, EC 3.1.4.5). In particular, it hydrolyses thermally denatured DNA more rapidly than native DNA." @default.
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- W2018234638 date "1964-09-01" @default.
- W2018234638 modified "2023-09-27" @default.
- W2018234638 title "The inhibition of DNA polymerase by extracts of rat liver partial purification and properties of a deoxyribonuclease" @default.
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- W2018234638 doi "https://doi.org/10.1016/0926-6550(64)90169-0" @default.
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