FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2018255715> ?p ?o ?g. }

• W2018255715 endingPage "321" @default.
• W2018255715 startingPage "313" @default.
• W2018255715 abstract "Human bone marrow-derived mesenchymal stem cells have the potential to differentiate into several cell types such as osteoblasts, chondrocytes, and adipocytes. When cultured under appropriate medium conditions stem cells can be directed toward the osteoblast lineage in vitro. Progression of osteogenic differentiation is accompanied by changes in the expression pattern of several marker proteins including bone-specific alkaline phosphatase (bALP), collagen I (Col I), and osteocalcin (OC) and can be analyzed by well-established methods like immunohistochemical staining and quantitative RT-PCR. Furthermore, expression of fluorescent protein driven by an osteogenesis promoter facilitates online monitoring of proceeding osteogenic differentiation in transiently transfected human bone marrow-derived cells. In the present study we established a new double reporter gene construct comprising OC promoter-driven expression of green fluorescent protein and constitutive expression of red fluorescent protein-tagged histone H2B for transient transfection of primary human bone cells (HBCs). Osteogenic differentiation of transiently transfected cells was visualized by fluorescence microscopy. Immunohistochemical analysis and RT-PCR confirmed the progression into the osteo-specific lineage of transfected cells. Transfection efficiency was determined by fluorescence-activated cell sorting (FACS)." @default.
• W2018255715 created "2016-06-24" @default.
• W2018255715 creator A5001283129 @default.
• W2018255715 creator A5021324674 @default.
• W2018255715 creator A5083527161 @default.
• W2018255715 date "2011-12-09" @default.
• W2018255715 modified "2023-10-10" @default.
• W2018255715 title "Watching osteogenesis: Life monitoring of osteogenic differentiation using an osteocalcin reporter" @default.
• W2018255715 cites W1566246481 @default.
• W2018255715 cites W1907841101 @default.
• W2018255715 cites W1967626549 @default.
• W2018255715 cites W1977950302 @default.
• W2018255715 cites W1983324401 @default.
• W2018255715 cites W1986276800 @default.
• W2018255715 cites W2012293496 @default.
• W2018255715 cites W2013076568 @default.
• W2018255715 cites W2019485891 @default.
• W2018255715 cites W2023680421 @default.
• W2018255715 cites W2035726898 @default.
• W2018255715 cites W2036276919 @default.
• W2018255715 cites W2038051362 @default.
• W2018255715 cites W2041507165 @default.
• W2018255715 cites W2050944929 @default.
• W2018255715 cites W2053687997 @default.
• W2018255715 cites W2060453748 @default.
• W2018255715 cites W2061412466 @default.
• W2018255715 cites W2070026305 @default.
• W2018255715 cites W2080452175 @default.
• W2018255715 cites W2081741520 @default.
• W2018255715 cites W2081896745 @default.
• W2018255715 cites W2087792566 @default.
• W2018255715 cites W2110148884 @default.
• W2018255715 cites W2116503335 @default.
• W2018255715 cites W2134208433 @default.
• W2018255715 cites W2136498711 @default.
• W2018255715 cites W2140812435 @default.
• W2018255715 cites W2144268541 @default.
• W2018255715 cites W2153042507 @default.
• W2018255715 cites W2169032395 @default.
• W2018255715 cites W4247889657 @default.
• W2018255715 doi "https://doi.org/10.1002/jcb.23357" @default.
• W2018255715 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21913216" @default.
• W2018255715 hasPublicationYear "2011" @default.
• W2018255715 type Work @default.
• W2018255715 sameAs 2018255715 @default.
• W2018255715 citedByCount "24" @default.
• W2018255715 countsByYear W20182557152012 @default.
• W2018255715 countsByYear W20182557152013 @default.
• W2018255715 countsByYear W20182557152014 @default.
• W2018255715 countsByYear W20182557152015 @default.
• W2018255715 countsByYear W20182557152016 @default.
• W2018255715 countsByYear W20182557152017 @default.
• W2018255715 countsByYear W20182557152018 @default.
• W2018255715 countsByYear W20182557152019 @default.
• W2018255715 countsByYear W20182557152021 @default.
• W2018255715 countsByYear W20182557152022 @default.
• W2018255715 crossrefType "journal-article" @default.
• W2018255715 hasAuthorship W2018255715A5001283129 @default.
• W2018255715 hasAuthorship W2018255715A5021324674 @default.
• W2018255715 hasAuthorship W2018255715A5083527161 @default.
• W2018255715 hasConcept C104317684 @default.
• W2018255715 hasConcept C131075544 @default.
• W2018255715 hasConcept C148738053 @default.
• W2018255715 hasConcept C153911025 @default.
• W2018255715 hasConcept C160160445 @default.
• W2018255715 hasConcept C181199279 @default.
• W2018255715 hasConcept C185592680 @default.
• W2018255715 hasConcept C198826908 @default.
• W2018255715 hasConcept C202751555 @default.
• W2018255715 hasConcept C203014093 @default.
• W2018255715 hasConcept C2778260815 @default.
• W2018255715 hasConcept C2778630268 @default.
• W2018255715 hasConcept C2780007613 @default.
• W2018255715 hasConcept C28328180 @default.
• W2018255715 hasConcept C54009773 @default.
• W2018255715 hasConcept C54355233 @default.
• W2018255715 hasConcept C553184892 @default.
• W2018255715 hasConcept C55493867 @default.
• W2018255715 hasConcept C81885089 @default.
• W2018255715 hasConcept C86803240 @default.
• W2018255715 hasConcept C95444343 @default.
• W2018255715 hasConceptScore W2018255715C104317684 @default.
• W2018255715 hasConceptScore W2018255715C131075544 @default.
• W2018255715 hasConceptScore W2018255715C148738053 @default.
• W2018255715 hasConceptScore W2018255715C153911025 @default.
• W2018255715 hasConceptScore W2018255715C160160445 @default.
• W2018255715 hasConceptScore W2018255715C181199279 @default.
• W2018255715 hasConceptScore W2018255715C185592680 @default.
• W2018255715 hasConceptScore W2018255715C198826908 @default.
• W2018255715 hasConceptScore W2018255715C202751555 @default.
• W2018255715 hasConceptScore W2018255715C203014093 @default.
• W2018255715 hasConceptScore W2018255715C2778260815 @default.
• W2018255715 hasConceptScore W2018255715C2778630268 @default.
• W2018255715 hasConceptScore W2018255715C2780007613 @default.
• W2018255715 hasConceptScore W2018255715C28328180 @default.
• W2018255715 hasConceptScore W2018255715C54009773 @default.
• W2018255715 hasConceptScore W2018255715C54355233 @default.
• W2018255715 hasConceptScore W2018255715C553184892 @default.

• next