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• W2018291226 abstract "We have developed a model of ischaemia-reperfusion injury in C57BL/6 mice involving ischaemia for 0.5 to 2.5 h with an elastic tourniquet on one hind limb and reperfusion for 24 h, analogous to a well-established model of ischaemia-reperfusion injury in the rat. Viability was assessed in tissue homogenates of the gastrocnemius muscles from the affected and contralateral control limb by a triphenyl tetrazolium chloride dye reaction, measuring the activity of the oxidative mitochondrial enzymes. After 1.5 h ischaemia and 24 h reperfusion, viability in the ischaemic-reperfused limb was 13%, with the control muscle regarded as 100% viable. Significant improvements in viability to 86% (P<0.05) and 56% (P<0.05) were achieved, with administration 30 min prior to tourniquet release, of the nitric oxide (NO) synthase inhibitor nitro-l-arginine methyl ester (l-NAME, 30 mg/kg) and the anti-inflammatory glucocorticoid dexamethasone (2.5 mg/kg) respectively, with similar findings in the rat tourniquet model." @default.
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• W2018291226 date "1997-08-01" @default.
• W2018291226 modified "2023-10-15" @default.
• W2018291226 title "Ischaemia-reperfusion injury in mouse skeletal muscle is reduced by Nω-nitro-l-arginine methyl ester and dexamethasone" @default.
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• W2018291226 doi "https://doi.org/10.1016/s0014-2999(97)01101-1" @default.
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