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• W2018341657 abstract "In this paper, a rapid and effective method based on capillary zone electrophoresis (CZE) coupled with electrospray ionization mass spectrometry (ESI-MS) was established for the trace analysis of microcystin (MC) isomers in crude algae sample. The experimental conditions including the composition, acidity and concentration of buffer, separation voltage, injection time, and MS detection parameters were investigated in detail. A capillary separation system was as follows: a uncoated fused-silica capillary tube (50 microm i.d. x 90 cm), 40 mmol L(-1) ammonium acetate solution (pH 9.86) as running buffer, 25 kV as separation voltage, 20 kV x 3s water first and 20 kV x 20s for sample injection. Mass analysis was performed in ESI source, with sheath gas temperature 150 degrees C, sheath gas pressure 10 psi, and sheath gas flow 6 L min(-1). And sheath liquid was 7.5 mmol L(-1) acetic acid in 50% isopropanol-water (3 microg L min(-1)). Protonation and ammonium adduct molecular ions m/z 506.9 (MC-LR) and 532.0 (MC-YR) were used for the quantification of MCs. Under these conditions, two MCs were baseline separated within 9 min, the calibration curves were obtained in the range of 0.11-10.0 microg mL(-1) and 0.16-10.5 microg mL(-1) for MC-LR and MC-YR, respectively. Meanwhile, limits of detection were 0.05 and 0.08 microg mL(-1) for MC-LR and MC-YR, respectively. The recoveries for the two MCs were in the range of 95.8-108%. The developed approach had been successfully applied to the analysis of MCs in crude algae samples." @default.
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• W2018341657 date "2010-09-01" @default.
• W2018341657 modified "2023-09-24" @default.
• W2018341657 title "Analysis of microcystins by capillary zone electrophoresis coupling with electrospray ionization mass spectrometry" @default.
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• W2018341657 doi "https://doi.org/10.1016/j.talanta.2010.05.045" @default.
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