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• W2018343044 abstract "Mice used in these tests were young adult males and females of generally uniform size. Cercariae of S. japonicum were obtained from naturally infected Oncomelania nosophora snails that were collected in an endemic center in Yamanashi Prefecture, Japan. Pools of cercariae from five or more infected snails were used for each of three tests. A sample of repellent cream was furnished by Dr. D. B. McMullen. The formula of the repellent as given by Bauman et al. (1955) is: 2% Rutgers 612, 2% Indalone and 6% dibutyl phthalate in a plasticized compound of silicone, nitrocellulose and castor oil in a vanishing cream base. In each of three tests, mice which had been prepared 24 hours previously by shaving their backs were divided into one control and two experimental groups. Approximately 0.1 gram of repellent cream was rubbed into the skin of the back of mice in the experimental groups; control mice were not treated with the repellent. At specified times thereafter each mouse was exposed to 30 cercariae that had been counted out accurately into watch glasses containing about 0.5 ml. of water. An effort was made to insure complete exposure by repeatedly touching the back of the mouse to the surface of the cercarial suspension. This was continued until cercariae could no longer be observed in the watch glass. The timing of repellent application and exposure to cercariae was so arranged that all cercariae required for each test could be obtained from the same pool. After exposure the mouse was handheld until its back had dried. The control group and the two experimental groups were housed in separate cages. Mice were not maintained individually, nor were their activities restricted in any way. All mice were sacrificed 4 to 5 weeks after exposure to cercariae. Schistosomes were recovered by a perfusion technic combined with intensive examination of tissues. The perfusion technic used was essentially that of Pan and Hunter (1951) with the exception that pressure on the perfusing medium was controlled by bottled air instead of a syringe." @default.
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• W2018343044 date "1956-10-01" @default.
• W2018343044 modified "2023-09-24" @default.
• W2018343044 title "Efficacy of Abbott's Insect Repellent Cream (E4856) in Preventing Schistosoma japonicum Infections in Mice" @default.
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• W2018343044 doi "https://doi.org/10.2307/3274453" @default.
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