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• W2018366249 abstract "ABSTRACT Natural transformation in Streptococcus pneumoniae is regulated by a quorum-sensing system that acts through accumulation and sensing of a peptide pheromone (competence-stimulating peptide [CSP]) to control many competence-specific genes acting in DNA uptake, processing, and integration. The period of competence induced by CSP lasts only 15 min (quarter-height peak width). The recently identified regulator ComX is required for the CSP-dependent expression of many competence-specific genes that share an unusual consensus sequence (TACGAATA) at their promoter regions. To test the hypothesis that this regulator acts as a transient alternative sigma factor, ComX was purified from an Escherichia coli overexpression strain and core RNA polymerase was purified from a comX -deficient S. pneumoniae strain. The reconstituted ComX-polymerase holoenzyme produced transcripts for the competence-specific genes ssbB , cinA , cglA , celA , and dalA and was inhibited by anti-ComX antibody, but not by anti-σ 70 antibody. Western blotting using antibodies specific for ComX, σ 70 , and poly-His revealed a transient presence of ComX for a period of 15 to 20 min after CSP treatment, while RNA polymerase remained at a constant level and σ A remained between 60 and 125% of its normal level. ComX reached a molar ratio to RNA polymerase of at least 1.5. We conclude that ComX is unstable and acts as a competence-specific sigma factor." @default.
• W2018366249 created "2016-06-24" @default.
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• W2018366249 date "2003-01-01" @default.
• W2018366249 modified "2023-10-01" @default.
• W2018366249 title "Transient Association of an Alternative Sigma Factor, ComX, with RNA Polymerase during the Period of Competence for Genetic Transformation in <i>Streptococcus pneumoniae</i>" @default.
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• W2018366249 doi "https://doi.org/10.1128/jb.185.1.349-358.2003" @default.
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