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- W2018402054 abstract "During oögenesis the ovaries of the blowfly Phormia terraenovae pass through different developmental stages. The titre of free ecdysteoids measured in the haemolymph, as well as in the ovaries, peaked at stage 4(a), at 300 pmol/ml and 5.18 ± 0.55 pmol/ovarian pair, respectively. Furthermore, the ovarian content of free ecdysteroids reached different age-dependent maximal values. Measurements of free and conjugated ovarian ecdysteroids showed a decrease of the conjugated hormone until stage 3(b), accompanied by an increase of the free ecdysteroid titre. Beginning with stage 4(a) the free ecdysteroids were reduced, and the bound form was again accumulated. Stage 4(a) ovaries also released ecdysteroids into the medium during in vitro incubations. Investigations with high-performance liquid chromatography (HPLC) showed that before and after enzymatic hydrolysis 60–80% of the total immunoreactive material comigrated with 20-hydroxyecdysone and ecdysone. Lower and higher polarity products, present in all fractions tested, ranged between 10–20% of the total immunoreactive material. In order to investigate possible correlations between the variations of free ecdysteroid titres and the energy production necessary for oögenesis, the activity of glycogen phosphorylase a and a + b of the fat body was measured, based on developmental stages. The activity curves of the glycogen phosphorylase of the fat body are indeed directly correlated with the concentration profiles of free ecdysteroids in the haemolymph and ovaries and reach maximal values during ovarian stage 4(a)." @default.
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- W2018402054 title "Origin of ecdysteroids in females of the blowfly Phormia terraenovae and their relation to reproduction and energy metabolism" @default.
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- W2018402054 doi "https://doi.org/10.1016/0022-1910(89)90090-5" @default.
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