Matches in SemOpenAlex for { <https://semopenalex.org/work/W2018447273> ?p ?o ?g. }
Showing items 1 to 60 of
60
with 100 items per page.
- W2018447273 abstract "DNA damage-activated homodimer of PARP-1 binds to single-strand breaks and catalyzes the synthesis and transfer of negatively charged ADP-ribose polymers to nuclear protein acceptors, including itself. It also undergoes site-specific proteolysis during apoptosis. On the other hand, DNA-PK is a heterotrimeric enzyme that specifically binds to double-strand breaks and phosphorylates its target proteins. Because both DNA breaks and apoptosis are known to occur following irradiation, whole-body irradiation was administered to find out the temporal pattern and dose-response of PARP expression and the activity pattern of DNA-PK. To assess the temporal response, male Wistar rats were subjected to a radiation dose of 3Gy and killed at various time intervals (1-24 hours). Both the PARP activity and expression were enhanced 4 hours after irradiation. Fragmented PARP was not observed until 24 hours after irradiation. The differential expression at DNA-PK various doses (0.1-5.0 Gy) was examined. The maximum expression of PARP was noted at 1 Gy, whereas the activation of DNA-PK was maximally observed at 3 Gy. We did not observe any increase in the expression of PARP until the dose of 3Gy was reached, which contradicted the findings in previous in vitro reports of PARP activation at high radiation doses. DNA-PK, however, showed a dose-dependent increase. Our results indicate that although both the PARP and the DNA-PK are nuclear enzymes with similar roles, the activation of these enzymes is dependent on the dose, and any extrapolation of data from in vitro observations can lead to misinterpretation." @default.
- W2018447273 created "2016-06-24" @default.
- W2018447273 creator A5032183582 @default.
- W2018447273 creator A5041133805 @default.
- W2018447273 creator A5045110168 @default.
- W2018447273 date "2004-01-01" @default.
- W2018447273 modified "2023-10-16" @default.
- W2018447273 title "Poly (ADP-Ribose) Polymerase and DNA-Dependent Protein Kinase: Differential Activation In Vivo" @default.
- W2018447273 doi "https://doi.org/10.1615/jenvpathtoxoncol.v23.i1.40" @default.
- W2018447273 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14994994" @default.
- W2018447273 hasPublicationYear "2004" @default.
- W2018447273 type Work @default.
- W2018447273 sameAs 2018447273 @default.
- W2018447273 citedByCount "0" @default.
- W2018447273 crossrefType "journal-article" @default.
- W2018447273 hasAuthorship W2018447273A5032183582 @default.
- W2018447273 hasAuthorship W2018447273A5041133805 @default.
- W2018447273 hasAuthorship W2018447273A5045110168 @default.
- W2018447273 hasConcept C134935766 @default.
- W2018447273 hasConcept C143425029 @default.
- W2018447273 hasConcept C153911025 @default.
- W2018447273 hasConcept C182979987 @default.
- W2018447273 hasConcept C185592680 @default.
- W2018447273 hasConcept C190283241 @default.
- W2018447273 hasConcept C207001950 @default.
- W2018447273 hasConcept C54355233 @default.
- W2018447273 hasConcept C552990157 @default.
- W2018447273 hasConcept C55493867 @default.
- W2018447273 hasConcept C82381507 @default.
- W2018447273 hasConcept C86803240 @default.
- W2018447273 hasConceptScore W2018447273C134935766 @default.
- W2018447273 hasConceptScore W2018447273C143425029 @default.
- W2018447273 hasConceptScore W2018447273C153911025 @default.
- W2018447273 hasConceptScore W2018447273C182979987 @default.
- W2018447273 hasConceptScore W2018447273C185592680 @default.
- W2018447273 hasConceptScore W2018447273C190283241 @default.
- W2018447273 hasConceptScore W2018447273C207001950 @default.
- W2018447273 hasConceptScore W2018447273C54355233 @default.
- W2018447273 hasConceptScore W2018447273C552990157 @default.
- W2018447273 hasConceptScore W2018447273C55493867 @default.
- W2018447273 hasConceptScore W2018447273C82381507 @default.
- W2018447273 hasConceptScore W2018447273C86803240 @default.
- W2018447273 hasLocation W20184472731 @default.
- W2018447273 hasLocation W20184472732 @default.
- W2018447273 hasOpenAccess W2018447273 @default.
- W2018447273 hasPrimaryLocation W20184472731 @default.
- W2018447273 hasRelatedWork W1977315934 @default.
- W2018447273 hasRelatedWork W2004103693 @default.
- W2018447273 hasRelatedWork W2042577703 @default.
- W2018447273 hasRelatedWork W2043680807 @default.
- W2018447273 hasRelatedWork W2045466228 @default.
- W2018447273 hasRelatedWork W2135956341 @default.
- W2018447273 hasRelatedWork W2801224314 @default.
- W2018447273 hasRelatedWork W2888493183 @default.
- W2018447273 hasRelatedWork W2906776957 @default.
- W2018447273 hasRelatedWork W4206798030 @default.
- W2018447273 isParatext "false" @default.
- W2018447273 isRetracted "false" @default.
- W2018447273 magId "2018447273" @default.
- W2018447273 workType "article" @default.