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- W2018456465 abstract "Targeted genome editing with engineered nucleases has transformed the ability to introduce precise sequence modifications at almost any site within the genome. A major obstacle to probing the efficiency and consequences of genome editing is that no existing method enables the frequency of different editing events to be simultaneously measured across a cell population at any endogenous genomic locus. We have developed a method for quantifying individual genome-editing outcomes at any site of interest with single-molecule real-time (SMRT) DNA sequencing. We show that this approach can be applied at various loci using multiple engineered nuclease platforms, including transcription-activator-like effector nucleases (TALENs), RNA-guided endonucleases (CRISPR/Cas9), and zinc finger nucleases (ZFNs), and in different cell lines to identify conditions and strategies in which the desired engineering outcome has occurred. This approach offers a technique for studying double-strand break repair, facilitates the evaluation of gene-editing technologies, and permits sensitive quantification of editing outcomes in almost every experimental system used." @default.
- W2018456465 created "2016-06-24" @default.
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- W2018456465 date "2014-04-01" @default.
- W2018456465 modified "2023-10-14" @default.
- W2018456465 title "Quantifying Genome-Editing Outcomes at Endogenous Loci with SMRT Sequencing" @default.
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- W2018456465 doi "https://doi.org/10.1016/j.celrep.2014.02.040" @default.
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