FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2018465102> ?p ?o ?g. }

• W2018465102 endingPage "72" @default.
• W2018465102 startingPage "65" @default.
• W2018465102 abstract "A fast affinity method for the semi-preparative isolation of recombinant Protein G from E. coli cell lysate is proposed. Rigid, macroporous affinity discs based on a glycidyl methacrylate–co-ethylene dimethacrylate polymer were used as chromatographic supports. The specific ligands (here human immunoglobulin G, hIgG) were immobilized by the one-step reaction between native epoxy groups of the polymer surface and ϵ-amino groups of the IgG molecules. No intermediate spacer was necessary to reach full biological activity of the ligand. The globular affinity ligands are located directly on the pore wall surface and are thereby freely accessible to target molecules (here Protein G) migrating with the mobile phase through the pores. It is shown that the conditions chosen for the hIgG immobilization do not involve an active site of the protein and thus do not bias the formation of the affinity complex. Chromatographically determined constants of dissociation of hIgG–Protein G affinity complexes confirm the high selectivity of this separation method. Two different aspects of the affinity separation are discussed, which differ mostly in terms of scale. In disc chromatography, high volumetric flow velocities are possible because of the small backpressure. Since in addition the mass transfer is more efficient, it becomes possible to achieve very short analysis times. The discs proposed can be used in a single-step enrichment of Protein G from lysates of non-pathogenic E. coli. Gel electrophoresis data are used to demonstrate the high degree of purity achieved for the final product." @default.
• W2018465102 created "2016-06-24" @default.
• W2018465102 creator A5030045839 @default.
• W2018465102 creator A5061027783 @default.
• W2018465102 creator A5073129203 @default.
• W2018465102 creator A5008554100 @default.
• W2018465102 date "1998-03-01" @default.
• W2018465102 modified "2023-10-10" @default.
• W2018465102 title "Fast isolation of protein receptors from streptococci G by means of macroporous affinity discs" @default.
• W2018465102 cites W1504943018 @default.
• W2018465102 cites W1601256941 @default.
• W2018465102 cites W1775749144 @default.
• W2018465102 cites W1871146434 @default.
• W2018465102 cites W1980687301 @default.
• W2018465102 cites W1983584769 @default.
• W2018465102 cites W1986611205 @default.
• W2018465102 cites W1988708619 @default.
• W2018465102 cites W1994360923 @default.
• W2018465102 cites W1999137397 @default.
• W2018465102 cites W2000835330 @default.
• W2018465102 cites W2014871318 @default.
• W2018465102 cites W2028847693 @default.
• W2018465102 cites W2059105584 @default.
• W2018465102 cites W2061670429 @default.
• W2018465102 cites W2062508173 @default.
• W2018465102 cites W2063224156 @default.
• W2018465102 cites W2071236860 @default.
• W2018465102 cites W2078210658 @default.
• W2018465102 cites W2082596424 @default.
• W2018465102 cites W2083461635 @default.
• W2018465102 cites W2088928415 @default.
• W2018465102 cites W2090025822 @default.
• W2018465102 cites W2094364863 @default.
• W2018465102 cites W2104220801 @default.
• W2018465102 cites W4301316236 @default.
• W2018465102 doi "https://doi.org/10.1016/s0021-9673(97)00981-3" @default.
• W2018465102 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9542127" @default.
• W2018465102 hasPublicationYear "1998" @default.
• W2018465102 type Work @default.
• W2018465102 sameAs 2018465102 @default.
• W2018465102 citedByCount "72" @default.
• W2018465102 countsByYear W20184651022012 @default.
• W2018465102 countsByYear W20184651022014 @default.
• W2018465102 countsByYear W20184651022015 @default.
• W2018465102 countsByYear W20184651022020 @default.
• W2018465102 countsByYear W20184651022021 @default.
• W2018465102 countsByYear W20184651022022 @default.
• W2018465102 crossrefType "journal-article" @default.
• W2018465102 hasAuthorship W2018465102A5008554100 @default.
• W2018465102 hasAuthorship W2018465102A5030045839 @default.
• W2018465102 hasAuthorship W2018465102A5061027783 @default.
• W2018465102 hasAuthorship W2018465102A5073129203 @default.
• W2018465102 hasConcept C116569031 @default.
• W2018465102 hasConcept C118792377 @default.
• W2018465102 hasConcept C132050475 @default.
• W2018465102 hasConcept C159654299 @default.
• W2018465102 hasConcept C161790260 @default.
• W2018465102 hasConcept C166940927 @default.
• W2018465102 hasConcept C170493617 @default.
• W2018465102 hasConcept C178790620 @default.
• W2018465102 hasConcept C179247698 @default.
• W2018465102 hasConcept C181199279 @default.
• W2018465102 hasConcept C185592680 @default.
• W2018465102 hasConcept C203014093 @default.
• W2018465102 hasConcept C2779000548 @default.
• W2018465102 hasConcept C2780801859 @default.
• W2018465102 hasConcept C32909587 @default.
• W2018465102 hasConcept C40250595 @default.
• W2018465102 hasConcept C43617362 @default.
• W2018465102 hasConcept C521977710 @default.
• W2018465102 hasConcept C55493867 @default.
• W2018465102 hasConcept C57409179 @default.
• W2018465102 hasConcept C74998103 @default.
• W2018465102 hasConcept C86803240 @default.
• W2018465102 hasConcept C8985478 @default.
• W2018465102 hasConceptScore W2018465102C116569031 @default.
• W2018465102 hasConceptScore W2018465102C118792377 @default.
• W2018465102 hasConceptScore W2018465102C132050475 @default.
• W2018465102 hasConceptScore W2018465102C159654299 @default.
• W2018465102 hasConceptScore W2018465102C161790260 @default.
• W2018465102 hasConceptScore W2018465102C166940927 @default.
• W2018465102 hasConceptScore W2018465102C170493617 @default.
• W2018465102 hasConceptScore W2018465102C178790620 @default.
• W2018465102 hasConceptScore W2018465102C179247698 @default.
• W2018465102 hasConceptScore W2018465102C181199279 @default.
• W2018465102 hasConceptScore W2018465102C185592680 @default.
• W2018465102 hasConceptScore W2018465102C203014093 @default.
• W2018465102 hasConceptScore W2018465102C2779000548 @default.
• W2018465102 hasConceptScore W2018465102C2780801859 @default.
• W2018465102 hasConceptScore W2018465102C32909587 @default.
• W2018465102 hasConceptScore W2018465102C40250595 @default.
• W2018465102 hasConceptScore W2018465102C43617362 @default.
• W2018465102 hasConceptScore W2018465102C521977710 @default.
• W2018465102 hasConceptScore W2018465102C55493867 @default.
• W2018465102 hasConceptScore W2018465102C57409179 @default.
• W2018465102 hasConceptScore W2018465102C74998103 @default.
• W2018465102 hasConceptScore W2018465102C86803240 @default.
• W2018465102 hasConceptScore W2018465102C8985478 @default.

• next