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- W2018473603 abstract "A series of peptides of general primary structure (VSSLESK)n (n = 2, 3, 4, 5 and 6) were designed and synthesized by fluorenylmethyloxycarbonyl solid-phase synthesis using a convergent approach. Peptides containing 21, 28, 35 and 42 residues were modified with α-methoxy poly(ethylene glycol) (mPEG; mol. wt. 2000) by reaction of mPEG–succinimidyl carbonate with the α-amino group of the resin-attached protected peptides. The conformation and thermal stability of the peptides and of their AB block copolymers (A is the mPEG block, B the (VSSLESK)n block) in aqueous medium were investigated by circular dichroism, size-exclusion chromatography and by analytical ultracentrifugation. The helicity of peptides increased with increasing length in a cooperative manner. The peptides and mPEG–peptides with 35 and 42 amino acid residues (block copolymers) adopted a two-stranded α-helical coiled-coil conformation in aqueous solution. The presence of the polymer chain in the diblock hybrid copolymers had no disturbing effect with respect to the stability of the α-helical peptide part in these constructs. Moreover, the thermal stability of mPEG-modified 42-peptide was substantially higher than that of the native 42-peptide. Analytical ultracentrifugation data revealed that in phosphate-buffered saline solution (25–200 μM) the block copolymer mPEG-block-(VSSLESK)6 (PEG42) associated into stable intermolecular coiled-coil dimers. Thermal melting profiles of peptides and mPEG–peptides at concentration 0.4 g · L−1 in PBS. Molar ellipticity at 222 nm versus temperature. Heating rate 0.5 °C · min−1." @default.
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- W2018473603 date "2002-06-01" @default.
- W2018473603 modified "2023-10-17" @default.
- W2018473603 title "Associative diblock copolymers of poly(ethylene glycol) and coiled-coil peptides" @default.
- W2018473603 doi "https://doi.org/10.1002/1616-5195(200206)2:5<199::aid-mabi199>3.0.co;2-2" @default.
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