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- W2018493658 abstract "An assay with high selectivity and sensitivity has been developed which, for the first time, allows quantitative, simultaneous measurement in DNA of both 8-oxo-2'-deoxyguanosine (8-oxodG) and 8-oxo-2'-deoxyadenosine (8-oxodA)-important biomarkers of oxidative DNA damage in vivo. Using reversed-phase HPLC coupled to electrospray tandem mass spectrometry (HPLC-MS/MS) in multiple reaction monitoring (MRM) mode it was possible to detect background levels of these lesions in commercially available calf thymus DNA (85 +/- 3 and 7.1 +/- 0.2 per 10(6) DNA bases for 8-oxodG and 8-oxodA respectively; n = 3). Levels of 8-oxodG determined by HPLC coupled to an electrochemical detection system (HPLC-EC) were found to be similar (75 +/- 6 per 10(6) DNA bases; n = 3) to those obtained using tandem mass spectrometry." @default.
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- W2018493658 date "2000-11-01" @default.
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- W2018493658 title "Simultaneous Measurement of 8-Oxo-2′-deoxyguanosine and 8-Oxo-2′-deoxyadenosine by HPLC-MS/MS" @default.
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- W2018493658 doi "https://doi.org/10.1006/bbrc.2000.3752" @default.
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