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• W2018521331 abstract "The preparation and utilization of a novel particulate label based on fluorescent hybrid silica (FHS) nanoparticles are reported in this article. These nanoparticles have shown several unique advantages over existing dye molecules, quantum dots, and latex-based fluorescent particles in easy preparation, good photostability and high sensitivity. A high molar ratio of the fluorescent molecules present in the core to biomolecules on the particle surface was achieved by using the well-developed silica surface immobilization chemistry for biomolecular linking. A fluoroimmunoassay method for detecting trace level Hepatitis B Surface Antigen (HBsAg) was developed. The calibration graph for HBsAg was linear over the range 0.5–220 ng/ml with a detection limit of 0.1 ng/ml. The sensitivity is greatly increased when compared with the corresponding immunoassay performed with direct fluorophore labeling. The present work shows that these FHS nanoparticles are high-quality markers for biochemical assays." @default.
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• W2018521331 date "2004-02-01" @default.
• W2018521331 modified "2023-10-14" @default.
• W2018521331 title "Novel fluorescent silica nanoparticle probe for ultrasensitive immunoassays" @default.
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• W2018521331 doi "https://doi.org/10.1016/j.aca.2003.10.045" @default.
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