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- W2018521741 abstract "Oral infections of mice with Trichinella spiralis induce activation of peritoneal exudate cells to transiently express and secrete a crystallizable protein Ym1. Purification of Ym1 to homogeneity was achieved. It is a single chain polypeptide (45 kDa) with a strong tendency to crystallize at its isoelectric point (pI 5.7). Co-expression of Ym1 with Mac-1 and scavenger receptor pinpoints macrophages as its main producer. Protein microsequencing data provide information required for full-length cDNA cloning from libraries constructed from activated peritoneal exudate cells. A single open reading frame of 398 amino acids with a leader peptide (21 residues) typical of secretory protein was deduced and later deposited in GenBankTM (accession number M94584) in 1992. By means of surface plasmon resonance analyses, Ym1 has been shown to exhibit binding specificity to saccharides with a free amine group, such as GlcN, GalN, or GlcN polymers, but it failed to bind to other saccharides. The interaction is pH-dependent but Ca2+ and Mg2+ion-independent. The binding avidity of Ym1 to GlcN oligosaccharides was enhanced by more than 1000-fold due to the clustering effect. Specific binding of Ym1 to heparin suggests that heparin/heparan sulfate may be its physiological ligand in vivo during inflammation and/or tissue remodeling. Although it shares ∼30% homology with microbial chitinases, no chitinase activity was found associated with Ym1. Genomic Southern blot analyses suggest that Ym1 may represent a member of a novel lectin gene family. M94584,AAB62394" @default.
- W2018521741 created "2016-06-24" @default.
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- W2018521741 date "2001-05-01" @default.
- W2018521741 modified "2023-10-06" @default.
- W2018521741 title "A Macrophage Protein, Ym1, Transiently Expressed during Inflammation Is a Novel Mammalian Lectin" @default.
- W2018521741 cites W1482559745 @default.
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- W2018521741 cites W1492834548 @default.
- W2018521741 cites W1493485729 @default.
- W2018521741 cites W1522837765 @default.
- W2018521741 cites W1568609804 @default.
- W2018521741 cites W1574789459 @default.
- W2018521741 cites W1606162058 @default.
- W2018521741 cites W1606423922 @default.
- W2018521741 cites W1772341111 @default.
- W2018521741 cites W1900312847 @default.
- W2018521741 cites W1927383279 @default.
- W2018521741 cites W1929591819 @default.
- W2018521741 cites W1932052686 @default.
- W2018521741 cites W1959298931 @default.
- W2018521741 cites W1966126000 @default.
- W2018521741 cites W1971540677 @default.
- W2018521741 cites W1975630826 @default.
- W2018521741 cites W1979837673 @default.
- W2018521741 cites W1979984378 @default.
- W2018521741 cites W1984808204 @default.
- W2018521741 cites W1998733027 @default.
- W2018521741 cites W2000149921 @default.
- W2018521741 cites W2001104014 @default.
- W2018521741 cites W2002956679 @default.
- W2018521741 cites W2003176514 @default.
- W2018521741 cites W2004727400 @default.
- W2018521741 cites W2007479564 @default.
- W2018521741 cites W2010645431 @default.
- W2018521741 cites W2015390110 @default.
- W2018521741 cites W2016483318 @default.
- W2018521741 cites W2017347652 @default.
- W2018521741 cites W2018820394 @default.
- W2018521741 cites W2019414596 @default.
- W2018521741 cites W2022225506 @default.
- W2018521741 cites W2028377819 @default.
- W2018521741 cites W2029080316 @default.
- W2018521741 cites W2029921422 @default.
- W2018521741 cites W2032118018 @default.
- W2018521741 cites W2032552530 @default.
- W2018521741 cites W2045348184 @default.
- W2018521741 cites W2049079346 @default.
- W2018521741 cites W2055320083 @default.
- W2018521741 cites W2061792951 @default.
- W2018521741 cites W2065909887 @default.
- W2018521741 cites W2075260321 @default.
- W2018521741 cites W2079743166 @default.
- W2018521741 cites W2091503771 @default.
- W2018521741 cites W2092085734 @default.
- W2018521741 cites W2093748890 @default.
- W2018521741 cites W2100837269 @default.
- W2018521741 cites W2102349489 @default.
- W2018521741 cites W2107983150 @default.
- W2018521741 cites W2108859348 @default.
- W2018521741 cites W2111104826 @default.
- W2018521741 cites W2111301002 @default.
- W2018521741 cites W2115048327 @default.
- W2018521741 cites W2115993724 @default.
- W2018521741 cites W2133111763 @default.
- W2018521741 cites W2138270253 @default.
- W2018521741 cites W2144192610 @default.
- W2018521741 cites W2144456776 @default.
- W2018521741 cites W2149200346 @default.
- W2018521741 cites W2160806937 @default.
- W2018521741 cites W2169829977 @default.
- W2018521741 cites W2170418572 @default.
- W2018521741 cites W2172220794 @default.
- W2018521741 cites W2214033675 @default.
- W2018521741 cites W2318390251 @default.
- W2018521741 cites W2326169151 @default.
- W2018521741 cites W2329281679 @default.
- W2018521741 cites W2368756114 @default.
- W2018521741 doi "https://doi.org/10.1074/jbc.m010417200" @default.
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