Matches in SemOpenAlex for { <https://semopenalex.org/work/W2018550649> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2018550649 endingPage "929" @default.
- W2018550649 startingPage "921" @default.
- W2018550649 abstract "Plant-pathogenic bacteria are the causative agents of diseases in important agricultural crops and ornamental plants. The severe economic burden of these diseases requires seeking new approaches for their control, particularly because phytopathogenic bacteria are often resistant to available treatments. The type II secretion (T2S) system is a key virulence factor used by major groups of phytopathogenic bacteria. The T2S machinery transports many hydrolytic enzymes responsible for degradation of the plant cell wall, thus enabling successful colonization and dissemination of the bacteria in the plant host. The genetic inactivation of the T2S system leads to loss of virulence, which strongly suggests that targeting the T2S could enable new treatments against plant-pathogenic bacteria. Accordingly, we have designed and optimized an assay to identify small-molecule inhibitors of the T2S system. This assay uses a double parametric output: measurement of bacterial growth and the enzymatic activity of cellulase, which is secreted via the T2S pathway in our model organism Dickeya dadantii. The assay was evaluated by screening natural extracts, culture filtrates isolated from rhizosphere bacteria, and a collection of pharmaceutically active compounds in LOPAC(1280). The calculated Z' values of 0.63, 0.63, and 0.58, respectively, strongly suggest that the assay is applicable for a high-throughput screening platform." @default.
- W2018550649 created "2016-06-24" @default.
- W2018550649 creator A5003699231 @default.
- W2018550649 creator A5009459650 @default.
- W2018550649 creator A5026049665 @default.
- W2018550649 creator A5026114513 @default.
- W2018550649 creator A5040077015 @default.
- W2018550649 creator A5054214042 @default.
- W2018550649 creator A5066127242 @default.
- W2018550649 creator A5074308925 @default.
- W2018550649 date "2013-09-01" @default.
- W2018550649 modified "2023-10-17" @default.
- W2018550649 title "Development of a Quantitative Assay Amenable for High-Throughput Screening to Target the Type II Secretion System for New Treatments against Plant-Pathogenic Bacteria" @default.
- W2018550649 cites W1490572822 @default.
- W2018550649 cites W1497424220 @default.
- W2018550649 cites W1572398018 @default.
- W2018550649 cites W1861372810 @default.
- W2018550649 cites W1991001144 @default.
- W2018550649 cites W2004745913 @default.
- W2018550649 cites W2010513968 @default.
- W2018550649 cites W2012896889 @default.
- W2018550649 cites W2061734018 @default.
- W2018550649 cites W2065915163 @default.
- W2018550649 cites W2075111660 @default.
- W2018550649 cites W2094288404 @default.
- W2018550649 cites W2112558684 @default.
- W2018550649 cites W2113284511 @default.
- W2018550649 cites W2117165918 @default.
- W2018550649 cites W2169768163 @default.
- W2018550649 cites W2328483020 @default.
- W2018550649 doi "https://doi.org/10.1177/1087057113485426" @default.
- W2018550649 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23580665" @default.
- W2018550649 hasPublicationYear "2013" @default.
- W2018550649 type Work @default.
- W2018550649 sameAs 2018550649 @default.
- W2018550649 citedByCount "3" @default.
- W2018550649 countsByYear W20185506492017 @default.
- W2018550649 countsByYear W20185506492018 @default.
- W2018550649 crossrefType "journal-article" @default.
- W2018550649 hasAuthorship W2018550649A5003699231 @default.
- W2018550649 hasAuthorship W2018550649A5009459650 @default.
- W2018550649 hasAuthorship W2018550649A5026049665 @default.
- W2018550649 hasAuthorship W2018550649A5026114513 @default.
- W2018550649 hasAuthorship W2018550649A5040077015 @default.
- W2018550649 hasAuthorship W2018550649A5054214042 @default.
- W2018550649 hasAuthorship W2018550649A5066127242 @default.
- W2018550649 hasAuthorship W2018550649A5074308925 @default.
- W2018550649 hasBestOaLocation W20185506491 @default.
- W2018550649 hasConcept C104317684 @default.
- W2018550649 hasConcept C130211564 @default.
- W2018550649 hasConcept C141603559 @default.
- W2018550649 hasConcept C156203801 @default.
- W2018550649 hasConcept C181199279 @default.
- W2018550649 hasConcept C2778883795 @default.
- W2018550649 hasConcept C49039625 @default.
- W2018550649 hasConcept C51323132 @default.
- W2018550649 hasConcept C523546767 @default.
- W2018550649 hasConcept C54355233 @default.
- W2018550649 hasConcept C55493867 @default.
- W2018550649 hasConcept C60987743 @default.
- W2018550649 hasConcept C86626682 @default.
- W2018550649 hasConcept C86803240 @default.
- W2018550649 hasConcept C89423630 @default.
- W2018550649 hasConceptScore W2018550649C104317684 @default.
- W2018550649 hasConceptScore W2018550649C130211564 @default.
- W2018550649 hasConceptScore W2018550649C141603559 @default.
- W2018550649 hasConceptScore W2018550649C156203801 @default.
- W2018550649 hasConceptScore W2018550649C181199279 @default.
- W2018550649 hasConceptScore W2018550649C2778883795 @default.
- W2018550649 hasConceptScore W2018550649C49039625 @default.
- W2018550649 hasConceptScore W2018550649C51323132 @default.
- W2018550649 hasConceptScore W2018550649C523546767 @default.
- W2018550649 hasConceptScore W2018550649C54355233 @default.
- W2018550649 hasConceptScore W2018550649C55493867 @default.
- W2018550649 hasConceptScore W2018550649C60987743 @default.
- W2018550649 hasConceptScore W2018550649C86626682 @default.
- W2018550649 hasConceptScore W2018550649C86803240 @default.
- W2018550649 hasConceptScore W2018550649C89423630 @default.
- W2018550649 hasIssue "8" @default.
- W2018550649 hasLocation W20185506491 @default.
- W2018550649 hasLocation W20185506492 @default.
- W2018550649 hasOpenAccess W2018550649 @default.
- W2018550649 hasPrimaryLocation W20185506491 @default.
- W2018550649 hasRelatedWork W1969959955 @default.
- W2018550649 hasRelatedWork W1970386319 @default.
- W2018550649 hasRelatedWork W1987020986 @default.
- W2018550649 hasRelatedWork W2018550649 @default.
- W2018550649 hasRelatedWork W2024180691 @default.
- W2018550649 hasRelatedWork W2170772601 @default.
- W2018550649 hasRelatedWork W2604918665 @default.
- W2018550649 hasRelatedWork W3087957996 @default.
- W2018550649 hasRelatedWork W4210579874 @default.
- W2018550649 hasRelatedWork W4289109525 @default.
- W2018550649 hasVolume "18" @default.
- W2018550649 isParatext "false" @default.
- W2018550649 isRetracted "false" @default.
- W2018550649 magId "2018550649" @default.
- W2018550649 workType "article" @default.