FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2018584432> ?p ?o ?g. }

• W2018584432 endingPage "3878" @default.
• W2018584432 startingPage "3870" @default.
• W2018584432 abstract "The transport of carbamate through the large subunit of carbamoyl phosphate synthetase (CPS) from Escherichia coli was investigated by molecular dynamics and site-directed mutagenesis. Carbamate, the product of the reaction involving ATP, bicarbonate, and ammonia, must be delivered from the site of formation to the site of utilization by traveling nearly 40 A within the enzyme. Potentials of mean force (PMF) calculations along the entire tunnel for the translocation of carbamate indicate that the tunnel is composed of three continuous water pockets and two narrow connecting parts, near Ala-23 and Gly-575. The two narrow parts render two free energy barriers of 6.7 and 8.4 kcal/mol, respectively. Three water pockets were filled with about 21, 9, and 9 waters, respectively, and the corresponding relative free energies of carbamate residing in these free energy minima are 5.8, 0, and 1.6 kcal/mol, respectively. The release of phosphate into solution at the site for the formation of carbamate allows the side chain of Arg-306 to rotate toward Glu-25, Glu-383, and Glu-604. This rotation is virtually prohibited by a barrier of at least 23 kcal/mol when phosphate remains bound. This conformational change not only opens the entrance of the tunnel but also shields the charge-charge repulsion from the three glutamate residues when carbamate passes through the tunnel. Two mutants, A23F and G575F, were designed to block the migration of carbamate through the narrowest parts of the carbamate tunnel. The mutants retained only 1.7% and 3.8% of the catalytic activity for the synthesis of carbamoyl phosphate relative to the wild type CPS, respectively." @default.
• W2018584432 created "2016-06-24" @default.
• W2018584432 creator A5000687843 @default.
• W2018584432 creator A5002277619 @default.
• W2018584432 creator A5021110394 @default.
• W2018584432 creator A5051732177 @default.
• W2018584432 creator A5066943052 @default.
• W2018584432 date "2010-02-26" @default.
• W2018584432 modified "2023-10-16" @default.
• W2018584432 title "Carbamate Transport in Carbamoyl Phosphate Synthetase: A Theoretical and Experimental Investigation" @default.
• W2018584432 cites W1771251144 @default.
• W2018584432 cites W1967974891 @default.
• W2018584432 cites W1969092472 @default.
• W2018584432 cites W1969836007 @default.
• W2018584432 cites W1973060079 @default.
• W2018584432 cites W1976499671 @default.
• W2018584432 cites W1977637187 @default.
• W2018584432 cites W1978864551 @default.
• W2018584432 cites W1982345335 @default.
• W2018584432 cites W1982356449 @default.
• W2018584432 cites W1983687097 @default.
• W2018584432 cites W1990780055 @default.
• W2018584432 cites W1992191884 @default.
• W2018584432 cites W1993077801 @default.
• W2018584432 cites W1995042715 @default.
• W2018584432 cites W1995908284 @default.
• W2018584432 cites W2001137003 @default.
• W2018584432 cites W2009526730 @default.
• W2018584432 cites W2011034421 @default.
• W2018584432 cites W2015461045 @default.
• W2018584432 cites W2018532993 @default.
• W2018584432 cites W2023271753 @default.
• W2018584432 cites W2026001461 @default.
• W2018584432 cites W2035687084 @default.
• W2018584432 cites W2036894128 @default.
• W2018584432 cites W2038083805 @default.
• W2018584432 cites W2042306120 @default.
• W2018584432 cites W2044961513 @default.
• W2018584432 cites W2052211030 @default.
• W2018584432 cites W2055442008 @default.
• W2018584432 cites W2055655263 @default.
• W2018584432 cites W2059844062 @default.
• W2018584432 cites W2060809301 @default.
• W2018584432 cites W2065280757 @default.
• W2018584432 cites W2067174909 @default.
• W2018584432 cites W2071389082 @default.
• W2018584432 cites W2072796170 @default.
• W2018584432 cites W2073623394 @default.
• W2018584432 cites W2080049549 @default.
• W2018584432 cites W2081604308 @default.
• W2018584432 cites W2082257635 @default.
• W2018584432 cites W2083461294 @default.
• W2018584432 cites W2085266415 @default.
• W2018584432 cites W2085397786 @default.
• W2018584432 cites W2085562885 @default.
• W2018584432 cites W2085818775 @default.
• W2018584432 cites W2086957099 @default.
• W2018584432 cites W2089744440 @default.
• W2018584432 cites W2090837462 @default.
• W2018584432 cites W2092451952 @default.
• W2018584432 cites W2092642545 @default.
• W2018584432 cites W2094887689 @default.
• W2018584432 cites W2097882723 @default.
• W2018584432 cites W2102537419 @default.
• W2018584432 cites W2103636724 @default.
• W2018584432 cites W2107029002 @default.
• W2018584432 cites W2112154906 @default.
• W2018584432 cites W2117332309 @default.
• W2018584432 cites W2120686999 @default.
• W2018584432 cites W2127272780 @default.
• W2018584432 cites W2131288855 @default.
• W2018584432 cites W2147993766 @default.
• W2018584432 cites W2152301430 @default.
• W2018584432 cites W2161500110 @default.
• W2018584432 cites W3104305066 @default.
• W2018584432 cites W56138765 @default.
• W2018584432 doi "https://doi.org/10.1021/ja910441v" @default.
• W2018584432 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2847351" @default.
• W2018584432 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20187643" @default.
• W2018584432 hasPublicationYear "2010" @default.
• W2018584432 type Work @default.
• W2018584432 sameAs 2018584432 @default.
• W2018584432 citedByCount "14" @default.
• W2018584432 countsByYear W20185844322013 @default.
• W2018584432 countsByYear W20185844322015 @default.
• W2018584432 countsByYear W20185844322016 @default.
• W2018584432 countsByYear W20185844322017 @default.
• W2018584432 countsByYear W20185844322018 @default.
• W2018584432 countsByYear W20185844322021 @default.
• W2018584432 crossrefType "journal-article" @default.
• W2018584432 hasAuthorship W2018584432A5000687843 @default.
• W2018584432 hasAuthorship W2018584432A5002277619 @default.
• W2018584432 hasAuthorship W2018584432A5021110394 @default.
• W2018584432 hasAuthorship W2018584432A5051732177 @default.
• W2018584432 hasAuthorship W2018584432A5066943052 @default.
• W2018584432 hasBestOaLocation W20185844322 @default.
• W2018584432 hasConcept C185592680 @default.
• W2018584432 hasConcept C2776382133 @default.

• next