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- W2018585562 abstract "φ6 is an enveloped dsRNA bacterial virus. Its segmented genome resides inside the virion associated polymerase complex which is formed by four proteins (P1, P2, P4 and P7) encoded by the viral L segment. Complete and incomplete polymerase complex particles can be produced using cDNA copies of this largest genome segment. We have analysed the capacity of the different purified particles to (1) package φ6 (+) sense genomic precursors and unspecific RNA, (2) synthesize (−) and (+) strands and (3) bind φ6 specific and unspecific RNAs. Both (−) and (+ strand synthesis polymerase activities were found to be associated with protein P2. In addition to complete particles, particles lacking protein P2 were found to package and protect genomic precursor ssRNAs. Protein P7 was needed for efficient packaging. Regulation and specificity of the packaging were found to be independent of P2. Particles composed of proteins P1 and P4 did not package or protect RNA but did bind φ6 genomic (+) strand RNAs. The three φ6 (+) strands bound in equal amounts to the particles when tested alone in a filter binding assay. In competition experiments they competed each other for binding, indicating that individual binding sites for the three genomic (+) strands do not exist. Differences in RNA binding competition among the four particles were observed, suggesting that packaging specificity is achieved by complex interactions of proteins and genomic (+) strand RNAs during the advancement of the packaging process after the initial binding events." @default.
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- W2018585562 date "1995-06-01" @default.
- W2018585562 modified "2023-09-24" @default.
- W2018585562 title "RNA Binding, Packaging and Polymerase Activities of the Different Incomplete Polymerase Complex Particles of dsRNA Bacteriophage φ6" @default.
- W2018585562 doi "https://doi.org/10.1006/jmbi.1995.0317" @default.
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