Matches in SemOpenAlex for { <https://semopenalex.org/work/W2018602019> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2018602019 endingPage "955" @default.
- W2018602019 startingPage "947" @default.
- W2018602019 abstract "In the efforts to find an anti-viral treatment for dengue, a simple tryptophan fluorescence-screening assay aimed at identifying dengue domain III envelope (EIII) protein inhibitors was developed. Residue Trp391 of EIII was used as an intrinsic probe to monitor the change in fluorescence of the tryptophan residue upon binding to a peptide. The analysis was based on the electron excitation at 280 nm and fluorescence emission at 300-400 nm of EIII, followed by quenching of fluorescence in the presence of potential peptidic inhibitors coded DS36wt, DS36opt, DN58wt and DN58opt. The present study found that the fluorescence of the recombinant EIII was quenched following the binding of DS36opt, DN58wt and DN58opt in a concentration-dependent manner. Since the λmax for emission remained unchanged, the effect was not due to a change in the environment of the tryptophan side chain. In contrast, a minimal fluorescence-quenching effect of DS36wt at 20 and 40 µM suggested that the DS36wt does not have any binding ability to EIII. This was supported by a simple native-page gel retardation assay that showed a band shift of EIII domain when incubated with DS36opt, DN58wt and DN58opt but not with DS36wt. We thus developed a low-cost and convenient spectrophotometric binding assay for the analysis of EIII-peptide interactions in a drug screening application." @default.
- W2018602019 created "2016-06-24" @default.
- W2018602019 creator A5007521164 @default.
- W2018602019 creator A5010681619 @default.
- W2018602019 creator A5039765640 @default.
- W2018602019 creator A5041966205 @default.
- W2018602019 creator A5048671573 @default.
- W2018602019 creator A5049257176 @default.
- W2018602019 creator A5052165127 @default.
- W2018602019 creator A5077588803 @default.
- W2018602019 creator A5078938102 @default.
- W2018602019 date "2014-01-01" @default.
- W2018602019 modified "2023-10-15" @default.
- W2018602019 title "Dengue Envelope Domain III-Peptide Binding Analysis <i>via</i> Tryptophan Fluorescence Quenching Assay" @default.
- W2018602019 cites W2024498680 @default.
- W2018602019 doi "https://doi.org/10.1248/cpb.c14-00165" @default.
- W2018602019 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25273053" @default.
- W2018602019 hasPublicationYear "2014" @default.
- W2018602019 type Work @default.
- W2018602019 sameAs 2018602019 @default.
- W2018602019 citedByCount "3" @default.
- W2018602019 countsByYear W20186020192017 @default.
- W2018602019 countsByYear W20186020192019 @default.
- W2018602019 countsByYear W20186020192022 @default.
- W2018602019 crossrefType "journal-article" @default.
- W2018602019 hasAuthorship W2018602019A5007521164 @default.
- W2018602019 hasAuthorship W2018602019A5010681619 @default.
- W2018602019 hasAuthorship W2018602019A5039765640 @default.
- W2018602019 hasAuthorship W2018602019A5041966205 @default.
- W2018602019 hasAuthorship W2018602019A5048671573 @default.
- W2018602019 hasAuthorship W2018602019A5049257176 @default.
- W2018602019 hasAuthorship W2018602019A5052165127 @default.
- W2018602019 hasAuthorship W2018602019A5077588803 @default.
- W2018602019 hasAuthorship W2018602019A5078938102 @default.
- W2018602019 hasBestOaLocation W20186020191 @default.
- W2018602019 hasConcept C121332964 @default.
- W2018602019 hasConcept C121745418 @default.
- W2018602019 hasConcept C12554922 @default.
- W2018602019 hasConcept C185592680 @default.
- W2018602019 hasConcept C2776706248 @default.
- W2018602019 hasConcept C2779281246 @default.
- W2018602019 hasConcept C2781338088 @default.
- W2018602019 hasConcept C515207424 @default.
- W2018602019 hasConcept C55493867 @default.
- W2018602019 hasConcept C62520636 @default.
- W2018602019 hasConcept C86803240 @default.
- W2018602019 hasConcept C91881484 @default.
- W2018602019 hasConceptScore W2018602019C121332964 @default.
- W2018602019 hasConceptScore W2018602019C121745418 @default.
- W2018602019 hasConceptScore W2018602019C12554922 @default.
- W2018602019 hasConceptScore W2018602019C185592680 @default.
- W2018602019 hasConceptScore W2018602019C2776706248 @default.
- W2018602019 hasConceptScore W2018602019C2779281246 @default.
- W2018602019 hasConceptScore W2018602019C2781338088 @default.
- W2018602019 hasConceptScore W2018602019C515207424 @default.
- W2018602019 hasConceptScore W2018602019C55493867 @default.
- W2018602019 hasConceptScore W2018602019C62520636 @default.
- W2018602019 hasConceptScore W2018602019C86803240 @default.
- W2018602019 hasConceptScore W2018602019C91881484 @default.
- W2018602019 hasIssue "10" @default.
- W2018602019 hasLocation W20186020191 @default.
- W2018602019 hasLocation W20186020192 @default.
- W2018602019 hasOpenAccess W2018602019 @default.
- W2018602019 hasPrimaryLocation W20186020191 @default.
- W2018602019 hasRelatedWork W1994239116 @default.
- W2018602019 hasRelatedWork W2029947695 @default.
- W2018602019 hasRelatedWork W2044742946 @default.
- W2018602019 hasRelatedWork W2049510987 @default.
- W2018602019 hasRelatedWork W2076623874 @default.
- W2018602019 hasRelatedWork W2077846290 @default.
- W2018602019 hasRelatedWork W2088410157 @default.
- W2018602019 hasRelatedWork W2149502868 @default.
- W2018602019 hasRelatedWork W2361778723 @default.
- W2018602019 hasRelatedWork W2379056585 @default.
- W2018602019 hasVolume "62" @default.
- W2018602019 isParatext "false" @default.
- W2018602019 isRetracted "false" @default.
- W2018602019 magId "2018602019" @default.
- W2018602019 workType "article" @default.