FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2018656904> ?p ?o ?g. }

• W2018656904 endingPage "2704" @default.
• W2018656904 startingPage "2691" @default.
• W2018656904 abstract "ABSTRACT The bitopic membrane protein VirB10 of the Agrobacterium VirB/VirD4 type IV secretion system (T4SS) undergoes a structural transition in response to sensing of ATP binding or hydrolysis by the channel ATPases VirD4 and VirB11. This transition, detectable as a change in protease susceptibility, is required for DNA substrate passage through the translocation channel. Here, we present evidence that DNA substrate engagement with VirD4 and VirB11 also is required for activation of VirB10. Several DNA substrates (oncogenic T-DNA and plasmids RSF1010 and pCloDF13) induced the VirB10 conformational change, each by mechanisms requiring relaxase processing at cognate oriT sequences. VirD2 relaxase deleted of its translocation signal or any of the characterized relaxases produced in the absence of cognate DNA substrates did not induce the structural transition. Translocated effector proteins, e.g., VirE2, VirE3, and VirF, also did not induce the transition. By mutational analyses, we supplied evidence that the N-terminal periplasmic loop of VirD4, in addition to its catalytic site, is essential for early-stage DNA substrate transfer and the VirB10 conformational change. Further studies of VirB11 mutants established that three T4SS-mediated processes, DNA transfer, protein transfer, and pilus production, can be uncoupled and that the latter two processes proceed independently of the VirB10 conformational change. Our findings support a general model whereby DNA ligand binding with VirD4 and VirB11 stimulates ATP binding/hydrolysis, which in turn activates VirB10 through a structural transition. This transition confers an open-channel configuration enabling passage of the DNA substrate to the cell surface." @default.
• W2018656904 created "2016-06-24" @default.
• W2018656904 creator A5018842385 @default.
• W2018656904 creator A5022808419 @default.
• W2018656904 creator A5061760126 @default.
• W2018656904 creator A5077884040 @default.
• W2018656904 date "2013-06-01" @default.
• W2018656904 modified "2023-10-17" @default.
• W2018656904 title "DNA Substrate-Induced Activation of the Agrobacterium VirB/VirD4 Type IV Secretion System" @default.
• W2018656904 cites W1512711110 @default.
• W2018656904 cites W1546895016 @default.
• W2018656904 cites W1549932090 @default.
• W2018656904 cites W1552257323 @default.
• W2018656904 cites W1558837374 @default.
• W2018656904 cites W1560509369 @default.
• W2018656904 cites W1565824582 @default.
• W2018656904 cites W1610064810 @default.
• W2018656904 cites W1791897171 @default.
• W2018656904 cites W1865563467 @default.
• W2018656904 cites W1892807652 @default.
• W2018656904 cites W1927823545 @default.
• W2018656904 cites W1967122502 @default.
• W2018656904 cites W1971912164 @default.
• W2018656904 cites W1973785296 @default.
• W2018656904 cites W1974154066 @default.
• W2018656904 cites W1976328080 @default.
• W2018656904 cites W1980405459 @default.
• W2018656904 cites W1980735881 @default.
• W2018656904 cites W1982904740 @default.
• W2018656904 cites W1989128783 @default.
• W2018656904 cites W1992159473 @default.
• W2018656904 cites W1992764122 @default.
• W2018656904 cites W1996034388 @default.
• W2018656904 cites W1999925338 @default.
• W2018656904 cites W2005564301 @default.
• W2018656904 cites W2016510254 @default.
• W2018656904 cites W2018856015 @default.
• W2018656904 cites W2023507166 @default.
• W2018656904 cites W2023784732 @default.
• W2018656904 cites W2026854348 @default.
• W2018656904 cites W2029987289 @default.
• W2018656904 cites W2041548140 @default.
• W2018656904 cites W2041975751 @default.
• W2018656904 cites W2043126144 @default.
• W2018656904 cites W2043219019 @default.
• W2018656904 cites W2045885573 @default.
• W2018656904 cites W2053552108 @default.
• W2018656904 cites W2060714981 @default.
• W2018656904 cites W2064783278 @default.
• W2018656904 cites W2066928538 @default.
• W2018656904 cites W2067481183 @default.
• W2018656904 cites W2067727942 @default.
• W2018656904 cites W2072374845 @default.
• W2018656904 cites W2086984305 @default.
• W2018656904 cites W2090313369 @default.
• W2018656904 cites W2093826863 @default.
• W2018656904 cites W2099258771 @default.
• W2018656904 cites W2100740117 @default.
• W2018656904 cites W2104879715 @default.
• W2018656904 cites W2106764265 @default.
• W2018656904 cites W2112257878 @default.
• W2018656904 cites W2115167678 @default.
• W2018656904 cites W2116273677 @default.
• W2018656904 cites W2117728545 @default.
• W2018656904 cites W2121897497 @default.
• W2018656904 cites W2124094854 @default.
• W2018656904 cites W2127524852 @default.
• W2018656904 cites W2127847106 @default.
• W2018656904 cites W2128705734 @default.
• W2018656904 cites W2135771899 @default.
• W2018656904 cites W2142902213 @default.
• W2018656904 cites W2143649761 @default.
• W2018656904 cites W2144626747 @default.
• W2018656904 cites W2144814664 @default.
• W2018656904 cites W2144831477 @default.
• W2018656904 cites W2147302347 @default.
• W2018656904 cites W2149859711 @default.
• W2018656904 cites W2152586487 @default.
• W2018656904 cites W2153885735 @default.
• W2018656904 cites W2155509510 @default.
• W2018656904 cites W2157528992 @default.
• W2018656904 cites W2160014629 @default.
• W2018656904 cites W2164152642 @default.
• W2018656904 cites W2166183038 @default.
• W2018656904 cites W2180864382 @default.
• W2018656904 cites W2252995131 @default.
• W2018656904 cites W79381975 @default.
• W2018656904 doi "https://doi.org/10.1128/jb.00114-13" @default.
• W2018656904 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3676061" @default.
• W2018656904 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23564169" @default.
• W2018656904 hasPublicationYear "2013" @default.
• W2018656904 type Work @default.
• W2018656904 sameAs 2018656904 @default.
• W2018656904 citedByCount "54" @default.
• W2018656904 countsByYear W20186569042013 @default.
• W2018656904 countsByYear W20186569042014 @default.
• W2018656904 countsByYear W20186569042015 @default.
• W2018656904 countsByYear W20186569042016 @default.

• next