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• W2018657560 endingPage "9222" @default.
• W2018657560 startingPage "9211" @default.
• W2018657560 abstract "ATP sensitive potassium (KATP) channels are composed of four copies of a pore-forming inward rectifying potassium channel (Kir6.1 or Kir6.2) and four copies of a sulfonylurea receptor (SUR1, SUR2A, or SUR2B) that surround the pore. SUR proteins are members of the ATP-binding cassette (ABC) superfamily of proteins. Binding of MgATP at the SUR nucleotide binding domains (NBDs) results in NBD dimerization, and hydrolysis of MgATP at the NBDs leads to channel opening. The SUR proteins also mediate interactions with KATP channel openers (KCOs) that activate the channel, with KCO binding and/or activation involving residues in the transmembrane helices and cytoplasmic loops of the SUR proteins. Because the cytoplasmic loops make extensive interactions with the NBDs, we hypothesized that the NBDs may also be involved in KCO binding. Here, we report nuclear magnetic resonance (NMR) spectroscopy studies that demonstrate a specific interaction of the KCO pinacidil with the first nucleotide binding domain (NBD1) from SUR2A, the regulatory SUR protein in cardiac KATP channels. Intrinsic tryptophan fluorescence titrations also demonstrate binding of pinacidil to SUR2A NBD1, and fluorescent nucleotide binding studies show that pinacidil binding increases the affinity of SUR2A NBD1 for ATP. In contrast, the KCO diazoxide does not interact with SUR2A NBD1 under the same conditions. NMR relaxation experiments and size exclusion chromatography indicate that SUR2A NBD1 is monomeric under the conditions used in drug binding studies. These studies identify additional binding sites for commonly used KCOs and provide a foundation for testing binding of drugs to the SUR NBDs." @default.
• W2018657560 created "2016-06-24" @default.
• W2018657560 creator A5006466935 @default.
• W2018657560 creator A5027410091 @default.
• W2018657560 creator A5061265570 @default.
• W2018657560 date "2012-11-01" @default.
• W2018657560 modified "2023-09-26" @default.
• W2018657560 title "NMR and Fluorescence Studies of Drug Binding to the First Nucleotide Binding Domain of SUR2A" @default.
• W2018657560 cites W1520645352 @default.
• W2018657560 cites W1555144527 @default.
• W2018657560 cites W1556366471 @default.
• W2018657560 cites W1571734887 @default.
• W2018657560 cites W1579569054 @default.
• W2018657560 cites W1901856024 @default.
• W2018657560 cites W1927723646 @default.
• W2018657560 cites W1964430829 @default.
• W2018657560 cites W1970696414 @default.
• W2018657560 cites W1971317954 @default.
• W2018657560 cites W1977017927 @default.
• W2018657560 cites W1977944550 @default.
• W2018657560 cites W1980347800 @default.
• W2018657560 cites W1989149550 @default.
• W2018657560 cites W1992045485 @default.
• W2018657560 cites W1994240328 @default.
• W2018657560 cites W1994348363 @default.
• W2018657560 cites W1994720978 @default.
• W2018657560 cites W1994910636 @default.
• W2018657560 cites W1995216190 @default.
• W2018657560 cites W1995645034 @default.
• W2018657560 cites W1995692151 @default.
• W2018657560 cites W2001758114 @default.
• W2018657560 cites W2007334529 @default.
• W2018657560 cites W2015967183 @default.
• W2018657560 cites W2016061230 @default.
• W2018657560 cites W2016192648 @default.
• W2018657560 cites W2017543298 @default.
• W2018657560 cites W2018566925 @default.
• W2018657560 cites W2022342405 @default.
• W2018657560 cites W2022937133 @default.
• W2018657560 cites W2030383714 @default.
• W2018657560 cites W2031632335 @default.
• W2018657560 cites W2033247282 @default.
• W2018657560 cites W2033349405 @default.
• W2018657560 cites W2035395088 @default.
• W2018657560 cites W2039793500 @default.
• W2018657560 cites W2040688084 @default.
• W2018657560 cites W2047205850 @default.
• W2018657560 cites W2047458120 @default.
• W2018657560 cites W2054161536 @default.
• W2018657560 cites W2055189403 @default.
• W2018657560 cites W2055382555 @default.
• W2018657560 cites W2061802839 @default.
• W2018657560 cites W2065283382 @default.
• W2018657560 cites W2066254017 @default.
• W2018657560 cites W2067067698 @default.
• W2018657560 cites W2069602336 @default.
• W2018657560 cites W2073574973 @default.
• W2018657560 cites W2080938154 @default.
• W2018657560 cites W2081616441 @default.
• W2018657560 cites W2082176639 @default.
• W2018657560 cites W2091881995 @default.
• W2018657560 cites W2092544746 @default.
• W2018657560 cites W2100551854 @default.
• W2018657560 cites W2106882534 @default.
• W2018657560 cites W2108706632 @default.
• W2018657560 cites W2111935912 @default.
• W2018657560 cites W2112534180 @default.
• W2018657560 cites W2117813164 @default.
• W2018657560 cites W2128676004 @default.
• W2018657560 cites W2129879061 @default.
• W2018657560 cites W2137015675 @default.
• W2018657560 cites W2137704498 @default.
• W2018657560 cites W2139404258 @default.
• W2018657560 cites W2147281292 @default.
• W2018657560 cites W2147706045 @default.
• W2018657560 cites W2150753478 @default.
• W2018657560 cites W2157393167 @default.
• W2018657560 cites W2161849629 @default.
• W2018657560 cites W2161983628 @default.
• W2018657560 cites W2169821755 @default.
• W2018657560 cites W2250052379 @default.
• W2018657560 cites W2324976743 @default.
• W2018657560 cites W2404439418 @default.
• W2018657560 cites W4235607015 @default.
• W2018657560 doi "https://doi.org/10.1021/bi301019e" @default.
• W2018657560 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23078514" @default.
• W2018657560 hasPublicationYear "2012" @default.
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