Matches in SemOpenAlex for { <https://semopenalex.org/work/W2018671116> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W2018671116 endingPage "2243" @default.
- W2018671116 startingPage "2239" @default.
- W2018671116 abstract "N-Nitrosodimethylamine (NDMA) and N-nitrosodiethylamine (NDEA) are widely occurring nitrosamines and require enzyme-catalyzed activation for their carcinogenic actions. The low Km forms of the enzyme are generally considered to be important in the activation of environmental carcinogens. In this work we examined the role of cytochrome P450IIE1--a constitutive enzyme that is also inducible by acetone, ethanol, fasting and other factors--in catalyzing the dealkylation and denitrosation of these two carcinogens. The experimentally determined Km value of NDMA demethylase depended upon the experimental conditions and was lower when lower protein concentrations were used. Low Km values of 15-20 microM were observed for NDMA demethylase with different preparations of microsomes. In the deethylation of NDEA, a low Km of approximately 40 microM was observed for both control and acetone-induced microsomes. Immunoinhibition studies indicated that P450IIE1 was responsible for almost all the low Km NDMA demethylase activity in acetone-induced microsomes and greater than 80% in control microsomes. This enzyme was also responsible for about three-quarters of the low Km NDEA deethylase activity in acetone-induced microsomes and about half in control microsomes. The denitrosation of NDMA and NDEA was inhibited to approximately the same extents as the dealkylation reactions under different experimental conditions, suggesting the involvement of the same enzyme and perhaps a common initial intermediate in these two types of reactions. The relevance of this work and its relationship to related information in the literature are discussed." @default.
- W2018671116 created "2016-06-24" @default.
- W2018671116 creator A5019194665 @default.
- W2018671116 creator A5086188593 @default.
- W2018671116 creator A5088009363 @default.
- W2018671116 date "1990-01-01" @default.
- W2018671116 modified "2023-09-25" @default.
- W2018671116 title "Roles of cytochrome P450IIE1 in the dealkylation and denitrosation of <i>N</i>-nitrosodimethylamine and <i>N</i>-nitrosodiethylamine in rat liver microsomes" @default.
- W2018671116 doi "https://doi.org/10.1093/carcin/11.12.2239" @default.
- W2018671116 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2265475" @default.
- W2018671116 hasPublicationYear "1990" @default.
- W2018671116 type Work @default.
- W2018671116 sameAs 2018671116 @default.
- W2018671116 citedByCount "98" @default.
- W2018671116 countsByYear W20186711162012 @default.
- W2018671116 countsByYear W20186711162013 @default.
- W2018671116 countsByYear W20186711162015 @default.
- W2018671116 countsByYear W20186711162016 @default.
- W2018671116 countsByYear W20186711162017 @default.
- W2018671116 countsByYear W20186711162018 @default.
- W2018671116 countsByYear W20186711162019 @default.
- W2018671116 countsByYear W20186711162020 @default.
- W2018671116 countsByYear W20186711162022 @default.
- W2018671116 countsByYear W20186711162023 @default.
- W2018671116 crossrefType "journal-article" @default.
- W2018671116 hasAuthorship W2018671116A5019194665 @default.
- W2018671116 hasAuthorship W2018671116A5086188593 @default.
- W2018671116 hasAuthorship W2018671116A5088009363 @default.
- W2018671116 hasConcept C104317684 @default.
- W2018671116 hasConcept C114246631 @default.
- W2018671116 hasConcept C161790260 @default.
- W2018671116 hasConcept C164361826 @default.
- W2018671116 hasConcept C181199279 @default.
- W2018671116 hasConcept C185592680 @default.
- W2018671116 hasConcept C2776524530 @default.
- W2018671116 hasConcept C2777395278 @default.
- W2018671116 hasConcept C2780750100 @default.
- W2018671116 hasConcept C2780768313 @default.
- W2018671116 hasConcept C2781364455 @default.
- W2018671116 hasConcept C41091548 @default.
- W2018671116 hasConcept C526171541 @default.
- W2018671116 hasConcept C55493867 @default.
- W2018671116 hasConcept C87644729 @default.
- W2018671116 hasConceptScore W2018671116C104317684 @default.
- W2018671116 hasConceptScore W2018671116C114246631 @default.
- W2018671116 hasConceptScore W2018671116C161790260 @default.
- W2018671116 hasConceptScore W2018671116C164361826 @default.
- W2018671116 hasConceptScore W2018671116C181199279 @default.
- W2018671116 hasConceptScore W2018671116C185592680 @default.
- W2018671116 hasConceptScore W2018671116C2776524530 @default.
- W2018671116 hasConceptScore W2018671116C2777395278 @default.
- W2018671116 hasConceptScore W2018671116C2780750100 @default.
- W2018671116 hasConceptScore W2018671116C2780768313 @default.
- W2018671116 hasConceptScore W2018671116C2781364455 @default.
- W2018671116 hasConceptScore W2018671116C41091548 @default.
- W2018671116 hasConceptScore W2018671116C526171541 @default.
- W2018671116 hasConceptScore W2018671116C55493867 @default.
- W2018671116 hasConceptScore W2018671116C87644729 @default.
- W2018671116 hasIssue "12" @default.
- W2018671116 hasLocation W20186711161 @default.
- W2018671116 hasLocation W20186711162 @default.
- W2018671116 hasOpenAccess W2018671116 @default.
- W2018671116 hasPrimaryLocation W20186711161 @default.
- W2018671116 hasRelatedWork W1972433077 @default.
- W2018671116 hasRelatedWork W1974103293 @default.
- W2018671116 hasRelatedWork W1993084845 @default.
- W2018671116 hasRelatedWork W2018671116 @default.
- W2018671116 hasRelatedWork W2051777729 @default.
- W2018671116 hasRelatedWork W2076458871 @default.
- W2018671116 hasRelatedWork W2105262783 @default.
- W2018671116 hasRelatedWork W2406850605 @default.
- W2018671116 hasRelatedWork W2186813338 @default.
- W2018671116 hasRelatedWork W2500219274 @default.
- W2018671116 hasVolume "11" @default.
- W2018671116 isParatext "false" @default.
- W2018671116 isRetracted "false" @default.
- W2018671116 magId "2018671116" @default.
- W2018671116 workType "article" @default.