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• W2018672744 abstract "Iron toxicity may contribute to oxidative injury in cells surrounding an intracerebral haematoma. Cells detoxify iron by sequestering it in ferritin, a 24-mer heteropolymer constructed of H and L subunits. The relative antioxidant efficacy of H- and L-ferritin has not been defined and was tested in this study using an established model of hemin toxicity. Consistent with prior observations, cultures treated with 30 microM hemin sustained loss of approximately half of the cells by 6 h, as measured by LDH and MTT assays, and a 14-fold increase in protein carbonyls. Increasing expression of either ferritin by adenoviral gene transfer prior to hemin treatment had a similar protective effect. Quenching of calcein fluorescence, a marker of the labile iron pool, in hemin-treated cultures was also equally reduced by either subunit. These results suggest that over-expression of either H- or L-ferritin protects astrocytes from hemin and may be beneficial after CNS haemorrhage." @default.
• W2018672744 created "2016-06-24" @default.
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• W2018672744 date "2009-01-01" @default.
• W2018672744 modified "2023-09-26" @default.
• W2018672744 title "Increasing expression of H- or L-ferritin protects cortical astrocytes from hemin toxicity" @default.
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• W2018672744 doi "https://doi.org/10.1080/10715760902942808" @default.
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