FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2018692812> ?p ?o ?g. }

• W2018692812 endingPage "860" @default.
• W2018692812 startingPage "851" @default.
• W2018692812 abstract "We investigated the properties of metacyclic trypomastigotes of non-virulent Trypanosoma cruzi clone CL-14, as compared to the parental isolate CL. In contrast to the CL isolate, which produces high parasitemias in mice, metacyclic forms of clone CL-14 failed to produce patent infection. In vitro, the number of clone CL-14 parasites that entered epithelial HeLa cells, after 1 h incubation, was approximately four-fold lower than that of the CL isolate and at 72 h post-infection intracellular replication was not apparent whereas cells infected with the CL isolate contained large number of parasites replicating as amastigotes. CL isolate metacyclic forms were long and slender, with the kinetoplast localised closer to the nucleus than to the posterior end, whereas clone CL-14 parasites were shorter, with the kinetoplast very close to the posterior end. Cysteine proteinase cruzipain and trans-sialidase activities were lower in CL isolate than in clone CL-14. The surface profile was similar, except that the expression of gp82, the stage-specific glycoprotein that promotes CL isolate mucosal infection in vivo and host cell invasion in vitro, was greatly reduced on the surface of clone CL-14 metacyclic forms. Genistein, a specific inhibitor of protein tyrosine kinase, which is activated in CL isolate by binding of gp82 to its host cell receptor, did not affect host cell entry of clone CL-14. In contrast with CL isolate, the infectivity of clone CL-14 was not affected by phospholipase C inhibitor U73122 but was diminished by a combination of ionomycin plus NH(4)Cl, which releases Ca(2+) from acidic vacuoles. Internalisation of clone CL-14, but not of CL isolate, was significantly increased by treating parasites with neuraminidase, which removes sialic acid from the mucin-like surface molecule gp35/50. Taken together, our data suggest an association between the non-virulence of clone CL-14 metacyclic forms and the reduced expression of gp82, which precludes the activation of signal transduction pathways leading to effective host cell invasion." @default.
• W2018692812 created "2016-06-24" @default.
• W2018692812 creator A5011719356 @default.
• W2018692812 creator A5050848308 @default.
• W2018692812 creator A5055508464 @default.
• W2018692812 creator A5073860979 @default.
• W2018692812 creator A5091337932 @default.
• W2018692812 creator A5091788671 @default.
• W2018692812 date "2004-06-01" @default.
• W2018692812 modified "2023-10-02" @default.
• W2018692812 title "Molecular basis of non-virulence of Trypanosoma cruzi clone CL-14" @default.
• W2018692812 cites W1656644300 @default.
• W2018692812 cites W1875892240 @default.
• W2018692812 cites W1920782262 @default.
• W2018692812 cites W1956333353 @default.
• W2018692812 cites W1978189007 @default.
• W2018692812 cites W1980166477 @default.
• W2018692812 cites W1999317022 @default.
• W2018692812 cites W2002534697 @default.
• W2018692812 cites W2005287646 @default.
• W2018692812 cites W2005442970 @default.
• W2018692812 cites W2025835829 @default.
• W2018692812 cites W2026713605 @default.
• W2018692812 cites W2050769547 @default.
• W2018692812 cites W2062640365 @default.
• W2018692812 cites W2067965416 @default.
• W2018692812 cites W2068230933 @default.
• W2018692812 cites W2071410887 @default.
• W2018692812 cites W2085558419 @default.
• W2018692812 cites W2093239713 @default.
• W2018692812 cites W2100829476 @default.
• W2018692812 cites W2131346025 @default.
• W2018692812 cites W2133101176 @default.
• W2018692812 cites W2138931722 @default.
• W2018692812 cites W2143887849 @default.
• W2018692812 cites W2144622612 @default.
• W2018692812 cites W2147329362 @default.
• W2018692812 cites W2157292325 @default.
• W2018692812 cites W2165564070 @default.
• W2018692812 cites W2404924867 @default.
• W2018692812 cites W2411155873 @default.
• W2018692812 doi "https://doi.org/10.1016/j.ijpara.2004.03.003" @default.
• W2018692812 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15157768" @default.
• W2018692812 hasPublicationYear "2004" @default.
• W2018692812 type Work @default.
• W2018692812 sameAs 2018692812 @default.
• W2018692812 citedByCount "25" @default.
• W2018692812 countsByYear W20186928122012 @default.
• W2018692812 countsByYear W20186928122013 @default.
• W2018692812 countsByYear W20186928122014 @default.
• W2018692812 countsByYear W20186928122016 @default.
• W2018692812 countsByYear W20186928122017 @default.
• W2018692812 countsByYear W20186928122021 @default.
• W2018692812 countsByYear W20186928122022 @default.
• W2018692812 crossrefType "journal-article" @default.
• W2018692812 hasAuthorship W2018692812A5011719356 @default.
• W2018692812 hasAuthorship W2018692812A5050848308 @default.
• W2018692812 hasAuthorship W2018692812A5055508464 @default.
• W2018692812 hasAuthorship W2018692812A5073860979 @default.
• W2018692812 hasAuthorship W2018692812A5091337932 @default.
• W2018692812 hasAuthorship W2018692812A5091788671 @default.
• W2018692812 hasConcept C104317684 @default.
• W2018692812 hasConcept C106358424 @default.
• W2018692812 hasConcept C136764020 @default.
• W2018692812 hasConcept C153911025 @default.
• W2018692812 hasConcept C159047783 @default.
• W2018692812 hasConcept C203014093 @default.
• W2018692812 hasConcept C2522874641 @default.
• W2018692812 hasConcept C2777625466 @default.
• W2018692812 hasConcept C2778048844 @default.
• W2018692812 hasConcept C2779121891 @default.
• W2018692812 hasConcept C2781092759 @default.
• W2018692812 hasConcept C3019824283 @default.
• W2018692812 hasConcept C41008148 @default.
• W2018692812 hasConcept C552990157 @default.
• W2018692812 hasConcept C55493867 @default.
• W2018692812 hasConcept C60987743 @default.
• W2018692812 hasConcept C71928629 @default.
• W2018692812 hasConcept C81089528 @default.
• W2018692812 hasConcept C86803240 @default.
• W2018692812 hasConcept C89423630 @default.
• W2018692812 hasConcept C98319499 @default.
• W2018692812 hasConceptScore W2018692812C104317684 @default.
• W2018692812 hasConceptScore W2018692812C106358424 @default.
• W2018692812 hasConceptScore W2018692812C136764020 @default.
• W2018692812 hasConceptScore W2018692812C153911025 @default.
• W2018692812 hasConceptScore W2018692812C159047783 @default.
• W2018692812 hasConceptScore W2018692812C203014093 @default.
• W2018692812 hasConceptScore W2018692812C2522874641 @default.
• W2018692812 hasConceptScore W2018692812C2777625466 @default.
• W2018692812 hasConceptScore W2018692812C2778048844 @default.
• W2018692812 hasConceptScore W2018692812C2779121891 @default.
• W2018692812 hasConceptScore W2018692812C2781092759 @default.
• W2018692812 hasConceptScore W2018692812C3019824283 @default.
• W2018692812 hasConceptScore W2018692812C41008148 @default.
• W2018692812 hasConceptScore W2018692812C552990157 @default.
• W2018692812 hasConceptScore W2018692812C55493867 @default.
• W2018692812 hasConceptScore W2018692812C60987743 @default.

• next