Matches in SemOpenAlex for { <https://semopenalex.org/work/W2018746585> ?p ?o ?g. }
Showing items 1 to 76 of
76
with 100 items per page.
- W2018746585 abstract "In this study we have assessed the action of a novel glycoprotease, secreted by the bovine pneumonia pathogen Pasteurella haemolytica, on epitectin expressed on the surface of human laryngeal carcinoma (H.Ep.2) cells. Epitectin has been previously characterized as a high buoyant density glycoprotein of mass of over 350 kDa extensively glycosylated on serine and threonine by small oligosaccharides. Purified metabolically labeled epitectin was very effectively hydrolyzed by the glycoprotease. However, short- and long-term treatments yielded a complex mixture of products which could not be resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) or column chromatography, probably because of the heterogeneity of the structure and the distribution of the saccharides. Treatment of H.Ep.2 cells with glycoprotease followed by flow cytometric analysis revealed a significant loss in the cell surface epitopes detected by the anti-epitectin Ca2 monoclonal antibody. The action of the glycoprotease on cell surface epitectin was blocked by anti-glycoprotease antisera and was absent in an extract of a glycoprotease-negative strain of P. haemolytica. When extracts of cells treated with glycoprotease for 4 h were subjected to SDS-PAGE followed by 125I-wheat germ agglutinin overlay and autoradiography, the intensity of the characteristic epitectin bands was found to be drastically reduced compared to controls. H.Ep.2 cells metabolically labeled with [3H]glucosamine were also incubated with or without the glycoprotease and the released products were fractionated and analyzed. The enzyme-released products were found to be enriched in mucin-type glycopeptides. Thus the P. haemolytica glycoprotease could be used to selectively degrade mucin glycoproteins on cancer cell surface." @default.
- W2018746585 created "2016-06-24" @default.
- W2018746585 creator A5032876883 @default.
- W2018746585 creator A5085129708 @default.
- W2018746585 creator A5089767696 @default.
- W2018746585 date "1994-05-01" @default.
- W2018746585 modified "2023-09-25" @default.
- W2018746585 title "Cleavage of Epitectin, a Mucin-Type Sialoglycoprotein, from the Surface of Human Laryngeal Carcinoma-Cells by a Glycoprotease from Pasteurella haemolytica" @default.
- W2018746585 doi "https://doi.org/10.1006/abbi.1994.1171" @default.
- W2018746585 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8179312" @default.
- W2018746585 hasPublicationYear "1994" @default.
- W2018746585 type Work @default.
- W2018746585 sameAs 2018746585 @default.
- W2018746585 citedByCount "10" @default.
- W2018746585 countsByYear W20187465852013 @default.
- W2018746585 countsByYear W20187465852014 @default.
- W2018746585 crossrefType "journal-article" @default.
- W2018746585 hasAuthorship W2018746585A5032876883 @default.
- W2018746585 hasAuthorship W2018746585A5085129708 @default.
- W2018746585 hasAuthorship W2018746585A5089767696 @default.
- W2018746585 hasConcept C101660505 @default.
- W2018746585 hasConcept C108625454 @default.
- W2018746585 hasConcept C116084860 @default.
- W2018746585 hasConcept C153911025 @default.
- W2018746585 hasConcept C159654299 @default.
- W2018746585 hasConcept C179247698 @default.
- W2018746585 hasConcept C179264091 @default.
- W2018746585 hasConcept C181199279 @default.
- W2018746585 hasConcept C185592680 @default.
- W2018746585 hasConcept C195616568 @default.
- W2018746585 hasConcept C203014093 @default.
- W2018746585 hasConcept C2776178622 @default.
- W2018746585 hasConcept C2778913249 @default.
- W2018746585 hasConcept C2780175098 @default.
- W2018746585 hasConcept C2780758401 @default.
- W2018746585 hasConcept C542903549 @default.
- W2018746585 hasConcept C55493867 @default.
- W2018746585 hasConcept C86803240 @default.
- W2018746585 hasConcept C89423630 @default.
- W2018746585 hasConceptScore W2018746585C101660505 @default.
- W2018746585 hasConceptScore W2018746585C108625454 @default.
- W2018746585 hasConceptScore W2018746585C116084860 @default.
- W2018746585 hasConceptScore W2018746585C153911025 @default.
- W2018746585 hasConceptScore W2018746585C159654299 @default.
- W2018746585 hasConceptScore W2018746585C179247698 @default.
- W2018746585 hasConceptScore W2018746585C179264091 @default.
- W2018746585 hasConceptScore W2018746585C181199279 @default.
- W2018746585 hasConceptScore W2018746585C185592680 @default.
- W2018746585 hasConceptScore W2018746585C195616568 @default.
- W2018746585 hasConceptScore W2018746585C203014093 @default.
- W2018746585 hasConceptScore W2018746585C2776178622 @default.
- W2018746585 hasConceptScore W2018746585C2778913249 @default.
- W2018746585 hasConceptScore W2018746585C2780175098 @default.
- W2018746585 hasConceptScore W2018746585C2780758401 @default.
- W2018746585 hasConceptScore W2018746585C542903549 @default.
- W2018746585 hasConceptScore W2018746585C55493867 @default.
- W2018746585 hasConceptScore W2018746585C86803240 @default.
- W2018746585 hasConceptScore W2018746585C89423630 @default.
- W2018746585 hasLocation W20187465851 @default.
- W2018746585 hasLocation W20187465852 @default.
- W2018746585 hasOpenAccess W2018746585 @default.
- W2018746585 hasPrimaryLocation W20187465851 @default.
- W2018746585 hasRelatedWork W1003977362 @default.
- W2018746585 hasRelatedWork W1496176043 @default.
- W2018746585 hasRelatedWork W2017816705 @default.
- W2018746585 hasRelatedWork W2018746585 @default.
- W2018746585 hasRelatedWork W2026887472 @default.
- W2018746585 hasRelatedWork W2036846953 @default.
- W2018746585 hasRelatedWork W2081233546 @default.
- W2018746585 hasRelatedWork W2081706092 @default.
- W2018746585 hasRelatedWork W2144992142 @default.
- W2018746585 hasRelatedWork W2172207865 @default.
- W2018746585 isParatext "false" @default.
- W2018746585 isRetracted "false" @default.
- W2018746585 magId "2018746585" @default.
- W2018746585 workType "article" @default.