Matches in SemOpenAlex for { <https://semopenalex.org/work/W2018749291> ?p ?o ?g. }
- W2018749291 endingPage "1107" @default.
- W2018749291 startingPage "1089" @default.
- W2018749291 abstract "Extensive measurements and analysis of thermodynamic stability and kinetics of urea-induced unfolding and folding of hisactophilin are reported for 5–50 °C, at pH 6.7. Under these conditions hisactophilin has moderate thermodynamic stability, and equilibrium and kinetic data are well fit by a two-state transition between the native and the denatured states. Equilibrium and kinetic m values decrease with increasing temperature, and decrease with increasing denaturant concentration. The βF values at different temperatures and urea concentrations are quite constant, however, at about 0.7. This suggests that the transition state for hisactophilin unfolding is native-like and changes little with changing solution conditions, consistent with a narrow free energy profile for the transition state. The activation enthalpy and entropy of unfolding are unusually low for hisactophilin, as is also the case for the corresponding equilibrium parameters. Conventional Arrhenius and Eyring plots for both folding and unfolding are markedly non-linear, but these plots become linear for constant ΔG/T contours. The Gibbs free energy changes for structural changes in hisactophilin have a non-linear denaturant dependence that is comparable to non-linearities observed for many other proteins. These non-linearities can be fit for many proteins using a variation of the Tanford model, incorporating empirical quadratic denaturant dependencies for Gibbs free energies of transfer of amino acid constituents from water to urea, and changes in fractional solvent accessible surface area of protein constituents based on the known protein structures. Noteworthy exceptions that are not well fit include amyloidogenic proteins and large proteins, which may form intermediates. The model is easily implemented and should be widely applicable to analysis of urea-induced structural transitions in proteins." @default.
- W2018749291 created "2016-06-24" @default.
- W2018749291 creator A5026132580 @default.
- W2018749291 creator A5040881026 @default.
- W2018749291 creator A5061503927 @default.
- W2018749291 creator A5067807269 @default.
- W2018749291 creator A5073722796 @default.
- W2018749291 date "2004-12-01" @default.
- W2018749291 modified "2023-10-14" @default.
- W2018749291 title "Non-linear Effects of Temperature and Urea on the Thermodynamics and Kinetics of Folding and Unfolding of Hisactophilin" @default.
- W2018749291 cites W1526967281 @default.
- W2018749291 cites W1554093892 @default.
- W2018749291 cites W1569015250 @default.
- W2018749291 cites W1575215071 @default.
- W2018749291 cites W1593987163 @default.
- W2018749291 cites W1605399271 @default.
- W2018749291 cites W1964576893 @default.
- W2018749291 cites W1967712722 @default.
- W2018749291 cites W1968291593 @default.
- W2018749291 cites W1968729198 @default.
- W2018749291 cites W1971358176 @default.
- W2018749291 cites W1971832114 @default.
- W2018749291 cites W1972272379 @default.
- W2018749291 cites W1972634563 @default.
- W2018749291 cites W1978583335 @default.
- W2018749291 cites W1978870059 @default.
- W2018749291 cites W1979056739 @default.
- W2018749291 cites W1979130049 @default.
- W2018749291 cites W1981583379 @default.
- W2018749291 cites W1983271858 @default.
- W2018749291 cites W1987918411 @default.
- W2018749291 cites W1988772022 @default.
- W2018749291 cites W1989297730 @default.
- W2018749291 cites W1989611394 @default.
- W2018749291 cites W1989919504 @default.
- W2018749291 cites W1991746427 @default.
- W2018749291 cites W1997539861 @default.
- W2018749291 cites W2000409271 @default.
- W2018749291 cites W2001812046 @default.
- W2018749291 cites W2002560825 @default.
- W2018749291 cites W2002967710 @default.
- W2018749291 cites W2005273145 @default.
- W2018749291 cites W2009857351 @default.
- W2018749291 cites W2011384774 @default.
- W2018749291 cites W2015746898 @default.
- W2018749291 cites W2015870840 @default.
- W2018749291 cites W2015901600 @default.
- W2018749291 cites W2017170609 @default.
- W2018749291 cites W2025026970 @default.
- W2018749291 cites W2031594282 @default.
- W2018749291 cites W2034246595 @default.
- W2018749291 cites W2037027088 @default.
- W2018749291 cites W2039154165 @default.
- W2018749291 cites W2040426346 @default.
- W2018749291 cites W2040522619 @default.
- W2018749291 cites W2042883952 @default.
- W2018749291 cites W2043778476 @default.
- W2018749291 cites W2044431641 @default.
- W2018749291 cites W2046915430 @default.
- W2018749291 cites W2048049130 @default.
- W2018749291 cites W2048629362 @default.
- W2018749291 cites W2048740018 @default.
- W2018749291 cites W2051063788 @default.
- W2018749291 cites W2053430992 @default.
- W2018749291 cites W2053942176 @default.
- W2018749291 cites W2056528113 @default.
- W2018749291 cites W2056831312 @default.
- W2018749291 cites W2061084111 @default.
- W2018749291 cites W2066196983 @default.
- W2018749291 cites W2067293617 @default.
- W2018749291 cites W2072746162 @default.
- W2018749291 cites W2074275590 @default.
- W2018749291 cites W2075699213 @default.
- W2018749291 cites W2075795921 @default.
- W2018749291 cites W2079227706 @default.
- W2018749291 cites W2080181966 @default.
- W2018749291 cites W2080844007 @default.
- W2018749291 cites W2081104286 @default.
- W2018749291 cites W2082176608 @default.
- W2018749291 cites W2082301993 @default.
- W2018749291 cites W2083143102 @default.
- W2018749291 cites W2083368946 @default.
- W2018749291 cites W2088165238 @default.
- W2018749291 cites W2091170915 @default.
- W2018749291 cites W2094799958 @default.
- W2018749291 cites W2095036416 @default.
- W2018749291 cites W2100814752 @default.
- W2018749291 cites W2102116608 @default.
- W2018749291 cites W2105774786 @default.
- W2018749291 cites W2110646423 @default.
- W2018749291 cites W2115592403 @default.
- W2018749291 cites W2116076986 @default.
- W2018749291 cites W2117747873 @default.
- W2018749291 cites W2121529915 @default.
- W2018749291 cites W2122625157 @default.
- W2018749291 cites W2138169394 @default.
- W2018749291 cites W2151837043 @default.
- W2018749291 cites W2153868467 @default.