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- W2018753752 abstract "A method for extracting and stabilizing a fish venom is proposed. Extracts from fresh venomous spines of Scorpaena guttata were prepared in distilled water at 5°, lyophilized, reconstituted in 0·05 M sodium phosphate buffer, pH 7·4, with 0·9 per cent NaCl and 10−3 M Cleland's reagent at 5°, and centrifuged. Using this technique, extracts were found to be stable up to 21 days. Fractionation indicated that the lethal property was associated with protein(s) having a molecular weight of more than 50,000 and less than 800,000. The semi-purified lethal fraction had an intravenous ld50 in mice of 0·9 mg protein per kg body weight, while the ld50 of the crude venom was 2·6 mg protein per kg. This is the first report on the separation of a fraction of a fish venom which is more lethal than the crude venom." @default.
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- W2018753752 date "1971-01-01" @default.
- W2018753752 modified "2023-10-10" @default.
- W2018753752 title "Some chemical properties of the venom of the scorpionfish Scorpaena guttata" @default.
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- W2018753752 doi "https://doi.org/10.1016/0041-0101(71)90045-6" @default.
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