Matches in SemOpenAlex for { <https://semopenalex.org/work/W2018766127> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2018766127 abstract "To study quantitatively the promoter methylation of hMLH1, p16INK, TIMP3 and TPEF genes in patients with colorectal cancer and synchronous polyps, and correlate it with some clinicomorphological features.DNA was extracted from all studied tumours and the corresponding normal mucosa. Microsatellite instability was analysed using two mononucleotide (BAT 25 and BAT 26) and three dinucleotide markers (D2S123, D5S356, D17S250) and automated DNA sequencing. Quantitative analysis of methylation was performed using DNA bisulfite modification, PCR with biotinylated primers, visualisation by 2% agarose gel electrophoresis and pyrosequencing.High methylation levels of hMLH1 and p16INK were found in elderly patients (mean age 73.8 +/- 9.5 years and 65.7 +/- 16.6 years, p < 0.03, t-test). Proximal tumours were more often associated with microsatellite instability (p < 0.05, Fisher's test) and higher level of methylation of hMLH1, p16INK and TIMP3 (p < 0.02, Kruskal-Wallis test), while tumours with poor differentiation tended to have higher methylation of the p16INK gene (p < 0.02, Kruskal-Wallis test). Local tumour invasion was correlated with the level of methylation of hMLH1, TIMP3 and the CpG island methylator phenotype (CIMP) status. Tumours with liver metastases showed a lower level of TIMP3 methylation than tumours with no systemic invasion (p < 0.05, Kruskal-Wallis test). We found concordance of methylation in 56% of the cases with colonic cancer and synchronous adenomas; the remaining 44% were discordant.Tumours with microsatellite instability, high level methylation and CIMP have distinctive clinical and morphological features. The level of hMLH1 and TIMP3 methylation and CIMP status can be correlated with the local tumour invasion. Different mechanisms, even for one and the same patient, can be responsible for the development of more than one third of the synchronous polyps and carcinomas." @default.
- W2018766127 created "2016-06-24" @default.
- W2018766127 creator A5017566788 @default.
- W2018766127 creator A5027658065 @default.
- W2018766127 creator A5060424972 @default.
- W2018766127 creator A5066376775 @default.
- W2018766127 creator A5088507934 @default.
- W2018766127 date "2010-01-01" @default.
- W2018766127 modified "2023-10-16" @default.
- W2018766127 title "DNA Methylation in Patients with Colorectal Cancer - Correlation with Some Clinical and Morphological Features and with Local Tumour Invasion" @default.
- W2018766127 cites W1579447084 @default.
- W2018766127 cites W1972600888 @default.
- W2018766127 cites W1975224807 @default.
- W2018766127 cites W1983339712 @default.
- W2018766127 cites W2002129512 @default.
- W2018766127 cites W2004440539 @default.
- W2018766127 cites W2005998569 @default.
- W2018766127 cites W2013807139 @default.
- W2018766127 cites W2019390139 @default.
- W2018766127 cites W2029484950 @default.
- W2018766127 cites W2030069314 @default.
- W2018766127 cites W2051584526 @default.
- W2018766127 cites W2053552125 @default.
- W2018766127 cites W2055732173 @default.
- W2018766127 cites W2100711594 @default.
- W2018766127 cites W2105428856 @default.
- W2018766127 cites W2107816687 @default.
- W2018766127 cites W2128146359 @default.
- W2018766127 cites W2130826348 @default.
- W2018766127 cites W2131829779 @default.
- W2018766127 doi "https://doi.org/10.2478/v10153-010-0043-9" @default.
- W2018766127 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20836393" @default.
- W2018766127 hasPublicationYear "2010" @default.
- W2018766127 type Work @default.
- W2018766127 sameAs 2018766127 @default.
- W2018766127 citedByCount "6" @default.
- W2018766127 countsByYear W20187661272012 @default.
- W2018766127 countsByYear W20187661272017 @default.
- W2018766127 countsByYear W20187661272019 @default.
- W2018766127 crossrefType "journal-article" @default.
- W2018766127 hasAuthorship W2018766127A5017566788 @default.
- W2018766127 hasAuthorship W2018766127A5027658065 @default.
- W2018766127 hasAuthorship W2018766127A5060424972 @default.
- W2018766127 hasAuthorship W2018766127A5066376775 @default.
- W2018766127 hasAuthorship W2018766127A5088507934 @default.
- W2018766127 hasConcept C104317684 @default.
- W2018766127 hasConcept C121608353 @default.
- W2018766127 hasConcept C140173407 @default.
- W2018766127 hasConcept C142724271 @default.
- W2018766127 hasConcept C150194340 @default.
- W2018766127 hasConcept C153911025 @default.
- W2018766127 hasConcept C160798450 @default.
- W2018766127 hasConcept C180754005 @default.
- W2018766127 hasConcept C190727270 @default.
- W2018766127 hasConcept C2779767149 @default.
- W2018766127 hasConcept C33288867 @default.
- W2018766127 hasConcept C502942594 @default.
- W2018766127 hasConcept C526805850 @default.
- W2018766127 hasConcept C54355233 @default.
- W2018766127 hasConcept C61320498 @default.
- W2018766127 hasConcept C71924100 @default.
- W2018766127 hasConcept C86803240 @default.
- W2018766127 hasConceptScore W2018766127C104317684 @default.
- W2018766127 hasConceptScore W2018766127C121608353 @default.
- W2018766127 hasConceptScore W2018766127C140173407 @default.
- W2018766127 hasConceptScore W2018766127C142724271 @default.
- W2018766127 hasConceptScore W2018766127C150194340 @default.
- W2018766127 hasConceptScore W2018766127C153911025 @default.
- W2018766127 hasConceptScore W2018766127C160798450 @default.
- W2018766127 hasConceptScore W2018766127C180754005 @default.
- W2018766127 hasConceptScore W2018766127C190727270 @default.
- W2018766127 hasConceptScore W2018766127C2779767149 @default.
- W2018766127 hasConceptScore W2018766127C33288867 @default.
- W2018766127 hasConceptScore W2018766127C502942594 @default.
- W2018766127 hasConceptScore W2018766127C526805850 @default.
- W2018766127 hasConceptScore W2018766127C54355233 @default.
- W2018766127 hasConceptScore W2018766127C61320498 @default.
- W2018766127 hasConceptScore W2018766127C71924100 @default.
- W2018766127 hasConceptScore W2018766127C86803240 @default.
- W2018766127 hasIssue "2" @default.
- W2018766127 hasLocation W20187661271 @default.
- W2018766127 hasLocation W20187661272 @default.
- W2018766127 hasOpenAccess W2018766127 @default.
- W2018766127 hasPrimaryLocation W20187661271 @default.
- W2018766127 hasRelatedWork W1558473757 @default.
- W2018766127 hasRelatedWork W2011831296 @default.
- W2018766127 hasRelatedWork W2029658031 @default.
- W2018766127 hasRelatedWork W2144936572 @default.
- W2018766127 hasRelatedWork W2612908078 @default.
- W2018766127 hasRelatedWork W2614320520 @default.
- W2018766127 hasRelatedWork W3109020289 @default.
- W2018766127 hasRelatedWork W3205214905 @default.
- W2018766127 hasRelatedWork W4386012301 @default.
- W2018766127 hasRelatedWork W4386045964 @default.
- W2018766127 hasVolume "52" @default.
- W2018766127 isParatext "false" @default.
- W2018766127 isRetracted "false" @default.
- W2018766127 magId "2018766127" @default.
- W2018766127 workType "article" @default.