FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2018777601> ?p ?o ?g. }

• W2018777601 endingPage "R74" @default.
• W2018777601 startingPage "R74" @default.
• W2018777601 abstract "Osteoarthritis is characterized by the progressive destruction of cartilage in the articular joints. Novel therapies that promote resurfacing of exposed bone in focal areas are of interest in osteoarthritis because they may delay the progression of this disabling disease in patients who develop focal lesions. Recently, the addition of 80% deacetylated chitosan to cartilage microfractures was shown to promote the regeneration of hyaline cartilage. The molecular mechanisms by which chitosan promotes cartilage regeneration remain unknown. Because neutrophils are transiently recruited to the microfracture site, the effect of 80% deacetylated chitosan on the function of neutrophils was investigated. Most studies on neutrophils use preparations of chitosan with an uncertain degree of deacetylation. For therapeutic purposes, it is of interest to determine whether the degree of deacetylation influences the response of neutrophils to chitosan. The effect of 95% deacetylated chitosan on the function of neutrophils was therefore also investigated and compared with that of 80% deacetylated chitosan. Human blood neutrophils from healthy donors were isolated by centrifugation on Ficoll-Paque. Chemotaxis was performed using the chemoTX system. Production of superoxide anions was evaluated using the cytochrome c reduction assay. Degranulation was determined by evaluating the release of myeloperoxidase and lactoferrin. The internalization of fluorescently labelled 80% deacetylated chitosan by neutrophils was studied by confocal microscopy. Neutrophils were dose dependently attracted to 80% deacetylated chitosan. In contrast, 95% deacetylated chitosan was not chemotactic for neutrophils. Moreover, the majority of the chemotactic effect of 80% deacetylated chitosan was mediated by phospholipase-A2-derived bioactive lipids. Contrary to the induction of chemotaxis, neither 80% nor 95% deacetylated chitosan activated the release of granule enzymes or the generation of active oxygen species. Despite the distinct response of neutrophils toward 80% and 95% deacetylated chitosan, both chitosans were internalized by neutrophils. Eighty per cent deacetylated chitosan induces a phenotype in neutrophils that is distinct from the classical phenotype induced by pro-inflammatory agents. Our observations also indicate that the degree of deacetylation is an important factor to consider in the use of chitosan as an accelerator of repair because neutrophils do not respond to 95% deacetylated chitosan." @default.
• W2018777601 created "2016-06-24" @default.
• W2018777601 creator A5018393891 @default.
• W2018777601 creator A5031774993 @default.
• W2018777601 creator A5040468356 @default.
• W2018777601 creator A5042838192 @default.
• W2018777601 creator A5045379753 @default.
• W2018777601 creator A5049465666 @default.
• W2018777601 creator A5062034330 @default.
• W2018777601 creator A5066358405 @default.
• W2018777601 date "2009-01-01" @default.
• W2018777601 modified "2023-10-17" @default.
• W2018777601 title "Neutrophils exhibit distinct phenotypes toward chitosans with different degrees of deacetylation: implications for cartilage repair" @default.
• W2018777601 cites W1486041456 @default.
• W2018777601 cites W1510401881 @default.
• W2018777601 cites W1973836626 @default.
• W2018777601 cites W1983509147 @default.
• W2018777601 cites W1994521826 @default.
• W2018777601 cites W2000645862 @default.
• W2018777601 cites W2010829192 @default.
• W2018777601 cites W2013430931 @default.
• W2018777601 cites W2015943769 @default.
• W2018777601 cites W2020358486 @default.
• W2018777601 cites W2029140668 @default.
• W2018777601 cites W2039451201 @default.
• W2018777601 cites W2040220172 @default.
• W2018777601 cites W2056970592 @default.
• W2018777601 cites W2064184046 @default.
• W2018777601 cites W2067270638 @default.
• W2018777601 cites W2070264145 @default.
• W2018777601 cites W2076816724 @default.
• W2018777601 cites W2083111869 @default.
• W2018777601 cites W2094124002 @default.
• W2018777601 cites W2098835923 @default.
• W2018777601 cites W2104948210 @default.
• W2018777601 cites W2106729611 @default.
• W2018777601 cites W2133529034 @default.
• W2018777601 cites W2152805338 @default.
• W2018777601 cites W2163000458 @default.
• W2018777601 cites W2163297145 @default.
• W2018777601 cites W2258795479 @default.
• W2018777601 cites W2319695212 @default.
• W2018777601 cites W395475022 @default.
• W2018777601 doi "https://doi.org/10.1186/ar2703" @default.
• W2018777601 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2714120" @default.
• W2018777601 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19460141" @default.
• W2018777601 hasPublicationYear "2009" @default.
• W2018777601 type Work @default.
• W2018777601 sameAs 2018777601 @default.
• W2018777601 citedByCount "43" @default.
• W2018777601 countsByYear W20187776012012 @default.
• W2018777601 countsByYear W20187776012013 @default.
• W2018777601 countsByYear W20187776012014 @default.
• W2018777601 countsByYear W20187776012015 @default.
• W2018777601 countsByYear W20187776012016 @default.
• W2018777601 countsByYear W20187776012017 @default.
• W2018777601 countsByYear W20187776012019 @default.
• W2018777601 countsByYear W20187776012020 @default.
• W2018777601 countsByYear W20187776012021 @default.
• W2018777601 countsByYear W20187776012022 @default.
• W2018777601 countsByYear W20187776012023 @default.
• W2018777601 crossrefType "journal-article" @default.
• W2018777601 hasAuthorship W2018777601A5018393891 @default.
• W2018777601 hasAuthorship W2018777601A5031774993 @default.
• W2018777601 hasAuthorship W2018777601A5040468356 @default.
• W2018777601 hasAuthorship W2018777601A5042838192 @default.
• W2018777601 hasAuthorship W2018777601A5045379753 @default.
• W2018777601 hasAuthorship W2018777601A5049465666 @default.
• W2018777601 hasAuthorship W2018777601A5062034330 @default.
• W2018777601 hasAuthorship W2018777601A5066358405 @default.
• W2018777601 hasBestOaLocation W20187776011 @default.
• W2018777601 hasConcept C105702510 @default.
• W2018777601 hasConcept C185592680 @default.
• W2018777601 hasConcept C203014093 @default.
• W2018777601 hasConcept C2779732960 @default.
• W2018777601 hasConcept C2780550940 @default.
• W2018777601 hasConcept C55493867 @default.
• W2018777601 hasConcept C71924100 @default.
• W2018777601 hasConceptScore W2018777601C105702510 @default.
• W2018777601 hasConceptScore W2018777601C185592680 @default.
• W2018777601 hasConceptScore W2018777601C203014093 @default.
• W2018777601 hasConceptScore W2018777601C2779732960 @default.
• W2018777601 hasConceptScore W2018777601C2780550940 @default.
• W2018777601 hasConceptScore W2018777601C55493867 @default.
• W2018777601 hasConceptScore W2018777601C71924100 @default.
• W2018777601 hasIssue "3" @default.
• W2018777601 hasLocation W20187776011 @default.
• W2018777601 hasLocation W20187776012 @default.
• W2018777601 hasLocation W20187776013 @default.
• W2018777601 hasLocation W20187776014 @default.
• W2018777601 hasLocation W20187776015 @default.
• W2018777601 hasOpenAccess W2018777601 @default.
• W2018777601 hasPrimaryLocation W20187776011 @default.
• W2018777601 hasRelatedWork W1531601525 @default.
• W2018777601 hasRelatedWork W2319480705 @default.
• W2018777601 hasRelatedWork W2384464875 @default.
• W2018777601 hasRelatedWork W2606230654 @default.
• W2018777601 hasRelatedWork W2607424097 @default.

• next