Matches in SemOpenAlex for { <https://semopenalex.org/work/W2018791839> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2018791839 endingPage "56" @default.
- W2018791839 startingPage "50" @default.
- W2018791839 abstract "The effect of the denaturants guanidine hydrochloride (GdnHCl) and urea on enzyme activity has been investigated. Inactivation of hen egg white lysozyme and bovine carbonic anhydrase was monitored by enzyme activity assays, and fluorescence spectroscopy was used to detect conformational changes. The results show that lysozyme and carbonic anhydrase were inactivated at GdnHCl and urea concentrations which were much lower than those required to cause denaturation. Ionic strength effects, examined by addition of NaCl, were found to significantly reduce the activity of lysozyme and carbonic anhydrase to different extents. Denaturant type was also found to affect refolding yields. The refolding of 0.015mgml-1 reduced, denatured lysozyme resulted in a maximum 65% recovery of activity from GdnHCl and 50% from urea. The first order rate constants for refolding were found to be 2.4×10-3 s-1 and 2.5×10-3 s-1 for GdnHCl and urea denatured lysozyme respectively. It is believed that the differences in yield from these two denaturants are due to their effect on protein aggregation. Concentrations of GdnHCl greater than 0.01M present during refolding greatly reduced the yield of active protein." @default.
- W2018791839 created "2016-06-24" @default.
- W2018791839 creator A5010881984 @default.
- W2018791839 creator A5076593394 @default.
- W2018791839 creator A5087682814 @default.
- W2018791839 date "1997-03-01" @default.
- W2018791839 modified "2023-09-25" @default.
- W2018791839 title "A Comparison of the Denaturants Urea and Guanidine Hydrochloride on Protein Refolding" @default.
- W2018791839 cites W1510728289 @default.
- W2018791839 cites W1519425272 @default.
- W2018791839 cites W1525083915 @default.
- W2018791839 cites W1562376901 @default.
- W2018791839 cites W1821376178 @default.
- W2018791839 cites W1857276803 @default.
- W2018791839 cites W1996112440 @default.
- W2018791839 cites W1999145924 @default.
- W2018791839 cites W2000209767 @default.
- W2018791839 cites W2006370393 @default.
- W2018791839 cites W2011808162 @default.
- W2018791839 cites W2013266980 @default.
- W2018791839 cites W2016402887 @default.
- W2018791839 cites W2021957452 @default.
- W2018791839 cites W2045667754 @default.
- W2018791839 cites W2077385058 @default.
- W2018791839 cites W2398317918 @default.
- W2018791839 cites W2483948949 @default.
- W2018791839 doi "https://doi.org/10.1205/096030897531360" @default.
- W2018791839 hasPublicationYear "1997" @default.
- W2018791839 type Work @default.
- W2018791839 sameAs 2018791839 @default.
- W2018791839 citedByCount "6" @default.
- W2018791839 countsByYear W20187918392013 @default.
- W2018791839 countsByYear W20187918392019 @default.
- W2018791839 countsByYear W20187918392020 @default.
- W2018791839 countsByYear W20187918392022 @default.
- W2018791839 countsByYear W20187918392023 @default.
- W2018791839 crossrefType "journal-article" @default.
- W2018791839 hasAuthorship W2018791839A5010881984 @default.
- W2018791839 hasAuthorship W2018791839A5076593394 @default.
- W2018791839 hasAuthorship W2018791839A5087682814 @default.
- W2018791839 hasConcept C185592680 @default.
- W2018791839 hasConcept C2776460385 @default.
- W2018791839 hasConcept C2779463914 @default.
- W2018791839 hasConcept C2780365088 @default.
- W2018791839 hasConcept C43617362 @default.
- W2018791839 hasConcept C55493867 @default.
- W2018791839 hasConceptScore W2018791839C185592680 @default.
- W2018791839 hasConceptScore W2018791839C2776460385 @default.
- W2018791839 hasConceptScore W2018791839C2779463914 @default.
- W2018791839 hasConceptScore W2018791839C2780365088 @default.
- W2018791839 hasConceptScore W2018791839C43617362 @default.
- W2018791839 hasConceptScore W2018791839C55493867 @default.
- W2018791839 hasIssue "1" @default.
- W2018791839 hasLocation W20187918391 @default.
- W2018791839 hasOpenAccess W2018791839 @default.
- W2018791839 hasPrimaryLocation W20187918391 @default.
- W2018791839 hasRelatedWork W1546487762 @default.
- W2018791839 hasRelatedWork W1988458827 @default.
- W2018791839 hasRelatedWork W2031764274 @default.
- W2018791839 hasRelatedWork W2040296201 @default.
- W2018791839 hasRelatedWork W2073883450 @default.
- W2018791839 hasRelatedWork W2152120207 @default.
- W2018791839 hasRelatedWork W2354066328 @default.
- W2018791839 hasRelatedWork W2400495362 @default.
- W2018791839 hasRelatedWork W2949979104 @default.
- W2018791839 hasRelatedWork W2188816439 @default.
- W2018791839 hasVolume "75" @default.
- W2018791839 isParatext "false" @default.
- W2018791839 isRetracted "false" @default.
- W2018791839 magId "2018791839" @default.
- W2018791839 workType "article" @default.