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- W2018828507 abstract "The etiologic evaluation of uveitis is frequently unsuccessful when noninvasive methods are used. We conducted a prospective study to evaluate systematic screening for pathogens of uveitis. All patients with uveitis referred to the participating tertiary ophthalmology departments from January 2001 to September 2007 underwent intraocular and serum specimen collection. The standardized protocol for laboratory investigations included universal polymerase chain reaction (PCR)-based detection of any bacteria and mycoses, specific PCR-based detection of fastidious (difficult-to-grow) bacteria and herpes viruses, and culture of vitreous fluid. Sera were tested for fastidious bacteria. Among the 1321 included patients (1520 specimens), infection was diagnosed in 147 (11.1%) patients: 78 (53%) were caused by fastidious bacteria that included spirochetes, Bartonella species, intracellular bacteria (Chlamydia species, Rickettsia species, Coxiella burnetii), and Tropheryma whipplei; 18 by herpes viruses; and 9 by fungi. Bartonella quintana, Coxiella burnetii, Paracoccus yeei, Aspergillus oryzae, and Cryptococcus albidus were found to be associated with uveitis for the first time, to our knowledge. We recommend applying a 1-step diagnostic procedure that incorporates intraocular, specific microbial PCR with serum analyses in tertiary centers to determine the etiology of uveitis. Abbreviations: ACP = anterior chamber paracentesis, EBV = Epstein-Barr virus, ELISA = enzyme-linked immunosorbent assay, HIV = human immunodeficiency virus, HSV = herpes simplex virus, PCR = polymerase chain reaction, TP-PA = Treponema pallidum particle agglutination test, VDRL = Venereal Disease Research Laboratory test." @default.
- W2018828507 created "2016-06-24" @default.
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- W2018828507 date "2008-05-01" @default.
- W2018828507 modified "2023-09-26" @default.
- W2018828507 title "High Prevalence of Fastidious Bacteria in 1520 Cases of Uveitis of Unknown Etiology" @default.
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- W2018828507 doi "https://doi.org/10.1097/md.0b013e31817b0747" @default.
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