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- W2018844955 abstract "Two pathogen-induced uridine diphosphate glycosyltransferases (UGTs) identified previously via co-expression with induced proanthocyanidin (PA) synthesis in poplar were cloned and characterized. Phylogenetic analysis grouped both genes with other known flavonoid UGTs that act on flavonols and anthocyanins. Recombinant enzymes were produced in order to test if they could glycoslate flavonoids. PtUGT78L1 accepted the flavonols quercetin and kaempferol as well as cyanidin, and used UDP-galactose as a sugar donor. PtUGT78M1 did not accept any of the flavonoids tested as a substrate, but did transfer glucose from UDP-glucose to the universal substrate 2,4,6-trichlorophenol. However, neither enzyme acted on the flavan-3-ols catechin or epicatechin, intermediates in the PA biosynthetic pathway." @default.
- W2018844955 created "2016-06-24" @default.
- W2018844955 creator A5010359005 @default.
- W2018844955 creator A5085689588 @default.
- W2018844955 date "2013-07-01" @default.
- W2018844955 modified "2023-09-27" @default.
- W2018844955 title "Molecular cloning and biochemical characterization of two UDP-glycosyltransferases from poplar" @default.
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- W2018844955 doi "https://doi.org/10.1016/j.phytochem.2012.12.012" @default.
- W2018844955 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23375153" @default.
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