Matches in SemOpenAlex for { <https://semopenalex.org/work/W2018872173> ?p ?o ?g. }
- W2018872173 endingPage "1766" @default.
- W2018872173 startingPage "1750" @default.
- W2018872173 abstract "Loss of cardiomyocytes impairs cardiac function after myocardial infarction (MI). Recent studies suggest that cardiac stem/progenitor cells could repair the damaged heart. However, cardiac progenitor cells are difficult to maintain in terms of purity and multipotency when propagated in two-dimensional culture systems. Here, we investigated a new strategy that enhances potency and enriches progenitor cells. We applied the repeated sphere formation strategy (cardiac explant → primary cardiosphere (CS) formation → sphere-derived cells (SDCs) in adherent culture condition → secondary CS formation by three-dimensional culture). Cells in secondary CS showed higher differentiation potentials than SDCs. When transplanted into the infarcted myocardium, secondary CSs engrafted robustly, improved left ventricular (LV) dysfunction, and reduced infarct sizes more than SDCs did. In addition to the cardiovascular differentiation of transplanted secondary CSs, robust vascular endothelial growth factor (VEGF) synthesis and secretion enhanced neovascularization in the infarcted myocardium. Microarray pathway analysis and blocking experiments using E-selectin knock-out hearts, specific chemicals, and small interfering RNAs (siRNAs) for each pathway revealed that E-selectin was indispensable to sphere initiation and ERK/Sp1/VEGF autoparacrine loop was responsible for sphere maturation. These results provide a simple strategy for enhancing cellular potency for cardiac repair. Furthermore, this strategy may be implemented to other types of stem/progenitor cell-based therapy." @default.
- W2018872173 created "2016-06-24" @default.
- W2018872173 creator A5005178090 @default.
- W2018872173 creator A5011828592 @default.
- W2018872173 creator A5018170954 @default.
- W2018872173 creator A5029552326 @default.
- W2018872173 creator A5032418747 @default.
- W2018872173 creator A5034370046 @default.
- W2018872173 creator A5037480434 @default.
- W2018872173 creator A5041904523 @default.
- W2018872173 creator A5051228518 @default.
- W2018872173 creator A5054506529 @default.
- W2018872173 creator A5055100242 @default.
- W2018872173 creator A5057429333 @default.
- W2018872173 creator A5057645077 @default.
- W2018872173 creator A5060029436 @default.
- W2018872173 creator A5073490917 @default.
- W2018872173 creator A5077238774 @default.
- W2018872173 date "2012-09-01" @default.
- W2018872173 modified "2023-10-14" @default.
- W2018872173 title "Secondary Sphere Formation Enhances the Functionality of Cardiac Progenitor Cells" @default.
- W2018872173 cites W1529396499 @default.
- W2018872173 cites W160771735 @default.
- W2018872173 cites W197648304 @default.
- W2018872173 cites W1980917009 @default.
- W2018872173 cites W1997694334 @default.
- W2018872173 cites W2007675851 @default.
- W2018872173 cites W2009485059 @default.
- W2018872173 cites W2010863589 @default.
- W2018872173 cites W2011648154 @default.
- W2018872173 cites W2021826108 @default.
- W2018872173 cites W2028036560 @default.
- W2018872173 cites W2029590548 @default.
- W2018872173 cites W2032815154 @default.
- W2018872173 cites W2036874650 @default.
- W2018872173 cites W2037424183 @default.
- W2018872173 cites W2045626197 @default.
- W2018872173 cites W2051868320 @default.
- W2018872173 cites W2055151337 @default.
- W2018872173 cites W2062847462 @default.
- W2018872173 cites W2067067950 @default.
- W2018872173 cites W2073607907 @default.
- W2018872173 cites W2078504009 @default.
- W2018872173 cites W2080539468 @default.
- W2018872173 cites W2086262311 @default.
- W2018872173 cites W2088561765 @default.
- W2018872173 cites W2088565743 @default.
- W2018872173 cites W2090807143 @default.
- W2018872173 cites W2104809829 @default.
- W2018872173 cites W2105956218 @default.
- W2018872173 cites W2106111979 @default.
- W2018872173 cites W2106251793 @default.
- W2018872173 cites W2112620963 @default.
- W2018872173 cites W2115980362 @default.
- W2018872173 cites W2122059700 @default.
- W2018872173 cites W2124396531 @default.
- W2018872173 cites W2128553876 @default.
- W2018872173 cites W2128923693 @default.
- W2018872173 cites W2140725738 @default.
- W2018872173 cites W2143839333 @default.
- W2018872173 cites W2148220540 @default.
- W2018872173 cites W2152387832 @default.
- W2018872173 cites W2159047813 @default.
- W2018872173 cites W2161041506 @default.
- W2018872173 cites W2161304727 @default.
- W2018872173 cites W2161368771 @default.
- W2018872173 cites W2169859308 @default.
- W2018872173 cites W2170215012 @default.
- W2018872173 cites W3149869420 @default.
- W2018872173 cites W4211016865 @default.
- W2018872173 doi "https://doi.org/10.1038/mt.2012.109" @default.
- W2018872173 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3437578" @default.
- W2018872173 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22713697" @default.
- W2018872173 hasPublicationYear "2012" @default.
- W2018872173 type Work @default.
- W2018872173 sameAs 2018872173 @default.
- W2018872173 citedByCount "37" @default.
- W2018872173 countsByYear W20188721732012 @default.
- W2018872173 countsByYear W20188721732013 @default.
- W2018872173 countsByYear W20188721732014 @default.
- W2018872173 countsByYear W20188721732015 @default.
- W2018872173 countsByYear W20188721732016 @default.
- W2018872173 countsByYear W20188721732017 @default.
- W2018872173 countsByYear W20188721732018 @default.
- W2018872173 countsByYear W20188721732019 @default.
- W2018872173 countsByYear W20188721732020 @default.
- W2018872173 countsByYear W20188721732021 @default.
- W2018872173 countsByYear W20188721732022 @default.
- W2018872173 countsByYear W20188721732023 @default.
- W2018872173 crossrefType "journal-article" @default.
- W2018872173 hasAuthorship W2018872173A5005178090 @default.
- W2018872173 hasAuthorship W2018872173A5011828592 @default.
- W2018872173 hasAuthorship W2018872173A5018170954 @default.
- W2018872173 hasAuthorship W2018872173A5029552326 @default.
- W2018872173 hasAuthorship W2018872173A5032418747 @default.
- W2018872173 hasAuthorship W2018872173A5034370046 @default.
- W2018872173 hasAuthorship W2018872173A5037480434 @default.
- W2018872173 hasAuthorship W2018872173A5041904523 @default.