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- W2018940122 abstract "Fifty strains belonging to Vibrio harveyi , Vibrio campbellii , and the recently described Vibrio rotiferianus , were analysed using phenotypic and genomic techniques with the aim of analysing the usefulness of the different techniques for the identification of V. harveyi -related species. The species V. harveyi and V. campbellii were phenotypically indistinguishable by more than 100 phenotypic features. Thirty-nine experimental strains were phenotypically identified as V. harveyi , but FAFLP, REP-PCR, IGS-PCR and DNA–DNA hybridization proved that they in fact belong to the species V. campbellii . Similar groupings were found among all fingerprinting methodologies (except IGS-PCR). Thirty-two experimental strains clustered with the V. campbellii type and one reference strain; seven strains clustered with the V. harveyi type and three reference strains; and the type and four reference strains of V. rotiferianus grouped together. The correlations between DNA–DNA hybridization and the genomic fingerprinting by FAFLP and (GTG) 5 -PCR were found to be above 0·68 and statistically significant, suggesting the value of the latter techniques for the reliable identification of V. harveyi -related species. The results presented indicate that strains phenotypically identified as V. harveyi are in fact V. campbellii ; these findings position V. campbellii as an important species involved in diseases of reared aquatic organisms." @default.
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- W2018940122 date "2004-06-01" @default.
- W2018940122 modified "2023-10-18" @default.
- W2018940122 title "Molecular identification of Vibrio harveyi-related isolates associated with diseased aquatic organisms" @default.
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- W2018940122 doi "https://doi.org/10.1099/mic.0.26797-0" @default.
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